Product nameAnti-CARHSP1 antibody
See all CARHSP1 primary antibodies
DescriptionRabbit polyclonal to CARHSP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to a region within amino acids 1-50 of Human CARHSP1 (NP_055131.2).
- 293T, HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab156694 was affinity purified using an epitope specific to CARHSP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab156694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 16 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionBinds mRNA and regulates the stability of target mRNA. Binds single-stranded DNA (in vitro).
Sequence similaritiesContains 1 CSD (cold-shock) domain.
modificationsDephosphorylated by calcineurin in a Ca(2+) dependent manner.
Cellular localizationCytoplasm. Cytoplasm > P-body. Cytoplasmic granule. Detected at cytoplasmic stress granules and P-bodies. Detected at exosome granules where mRNA is degraded.
- Information by UniProt
- Calcium regulated heat stable protein (24kD) antibody
- Calcium regulated heat stable protein 1 (24kD) antibody
- Calcium regulated heat stable protein 1 antibody
All lanes : Anti-CARHSP1 antibody (ab156694) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Predicted band size: 16 kDa
Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of CARHSP1 by Western blot of immunoprecipitate.
Immunoprecipitation of CARHSP1 (6 µg of antibody per mg of Jurkat whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: ab156694
Lane 2: alternative rabbit anti- CARHSP1 antibody
Lane 3: Control IgG
For the subsequent Western blot of immunoprecipitates, ab156694 was used at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab156694 has not yet been referenced specifically in any publications.