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Rabbit polyclonal to CARHSP1
Predicted to work with:
Synthetic peptide corresponding to a region within amino acids 1-50 of Human CARHSP1 (NP_055131.2).
293T, HeLa and Jurkat whole cell lysates.
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab156694 was affinity purified using an epitope specific to CARHSP1 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
Use at 2-10 µg/mg of lysate.
Binds mRNA and regulates the stability of target mRNA. Binds single-stranded DNA (in vitro).
Contains 1 CSD (cold-shock) domain.
Dephosphorylated by calcineurin in a Ca(2+) dependent manner.
Cytoplasm. Cytoplasm > P-body. Cytoplasmic granule. Detected at cytoplasmic stress granules and P-bodies. Detected at exosome granules where mRNA is degraded.
Information by UniProt
Calcium regulated heat stable protein (24kD) antibody
Calcium regulated heat stable protein 1 (24kD) antibody
Calcium regulated heat stable protein 1 antibody
Western blot - Anti-CARHSP1 antibody (ab156694)
All lanes : Anti-CARHSP1 antibody (ab156694) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Predicted band size: 16 kDa Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation - Anti-CARHSP1 antibody (ab156694)
Detection of CARHSP1 by Western blot of immunoprecipitate.
Immunoprecipitation of CARHSP1 (6 µg of antibody per mg of Jurkat whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: ab156694
Lane 2: alternative rabbit anti- CARHSP1 antibody
Lane 3: Control IgG
For the subsequent Western blot of immunoprecipitates, ab156694 was used at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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