Overview

  • Product name
  • Description
    Rabbit polyclonal to CARHSP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-50 of Human CARHSP1 (NP_055131.2).

  • Positive control
    • 293T, HeLa and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab156694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Binds mRNA and regulates the stability of target mRNA. Binds single-stranded DNA (in vitro).
  • Sequence similarities
    Contains 1 CSD (cold-shock) domain.
  • Post-translational
    modifications
    Dephosphorylated by calcineurin in a Ca(2+) dependent manner.
  • Cellular localization
    Cytoplasm. Cytoplasm > P-body. Cytoplasmic granule. Detected at cytoplasmic stress granules and P-bodies. Detected at exosome granules where mRNA is degraded.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium regulated heat stable protein (24kD) antibody
    • Calcium regulated heat stable protein 1 (24kD) antibody
    • Calcium regulated heat stable protein 1 antibody
    • Calcium-regulated heat stable protein 1 antibody
    • Calcium-regulated heat-stable protein of 24 kDa antibody
    • Carhsp1 antibody
    • CHSP1_HUMAN antibody
    • CRHSP-24 antibody
    • CRHSP24 antibody
    • CSDC1 antibody
    see all

Images

  • All lanes : Anti-CARHSP1 antibody (ab156694) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Predicted band size: 16 kDa



    Detection: Chemiluminescence with an exposure time of 30 seconds.
  • Detection of CARHSP1 by Western blot of immunoprecipitate.
    Immunoprecipitation of CARHSP1 (6 µg of antibody per mg of Jurkat whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
    Lane 1: ab156694
    Lane 2: alternative rabbit anti- CARHSP1 antibody
    Lane 3: Control IgG
    For the subsequent Western blot of immunoprecipitates, ab156694 was used at 1 µg/ml.
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab156694 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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