Anti-SHK antibody (ab69920)
Key features and details
- Rabbit polyclonal to SHK
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHK antibody
See all SHK primary antibodies -
Description
Rabbit polyclonal to SHK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human SHK (N terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
IHC-P
1/50 - 1/100. |
Target
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Tissue specificity
Strongly expressed in liver, kidney and pancreas. Expressed at lower levels in placenta and heart. Very weakly expressed in lung and brain. -
Sequence similarities
Belongs to the FGGY kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23729 Human
- Entrez Gene: 287479 Rat
- Omim: 605060 Human
- SwissProt: Q9UHJ6 Human
- Unigene: 579217 Human
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Alternative names
- Carbohydrate kinase like antibody
- Carbohydrate kinase like protein antibody
- Carbohydrate kinase-like protein antibody
see all
Images
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All lanes : Anti-SHK antibody (ab69920) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab69920 at 1/50 dilution, in the presence and absence of immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab69920 has been referenced in 3 publications.
- Ito Y et al. Identification of targets of tumor suppressor microRNA-34a using a reporter library system. Proc Natl Acad Sci U S A 114:3927-3932 (2017). PubMed: 28356515
- Wamelink MM et al. First two unrelated cases of isolated sedoheptulokinase deficiency: A benign disorder? J Inherit Metab Dis 38:889-94 (2015). WB . PubMed: 25647543
- Haschemi A et al. The sedoheptulose kinase CARKL directs macrophage polarization through control of glucose metabolism. Cell Metab 15:813-26 (2012). WB ; Mouse . PubMed: 22682222