Overview

  • Product name

  • Description

    Rabbit polyclonal to CARM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CARM1 aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab129212)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; MCF7; MDA MB 231; Jurkat; PC3; DU145 as well as HeLa Histone Nuclear. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: DI145.

Properties

Applications

Our Abpromise guarantee covers the use of ab128851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
  • Tissue specificity

    Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.
  • Post-translational
    modifications

    Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing.
    Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
  • Cellular localization

    Nucleus. Cytoplasm. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • carm1 antibody
    • CARM1_HUMAN antibody
    • Coactivator associated arginine methyltransferase 1 antibody
    • Coactivator-associated arginine methyltransferase 1 antibody
    • Histone arginine methyltransferase CARM 1 antibody
    • Histone arginine methyltransferase CARM1 antibody
    • Histone-arginine methyltransferase CARM1 antibody
    • PRMT 4 antibody
    • PRMT4 antibody
    • Protein arginine methyltransferase antibody
    • Protein arginine N methyltransferase 4 antibody
    • Protein arginine N-methyltransferase 4 antibody
    see all

Images

  • All lanes : Anti-CARM1 antibody (ab128851) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lane 4 : HeLa Histone Preparation Nuclear Lysate

    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lane 6 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
    Lane 7 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate


    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 66 kDa


    Exposure time: 30 seconds


    This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 3 of CARM1. The predicted molecular weights of isoforms 1 and 3 are 63 kDa and 66 kDa respectively.

  • ICC/IF image of ab128851 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab128851 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Li S  et al. The Overexpression of CARM1 Promotes Human Osteosarcoma Cell Proliferation through the pGSK3ß/ß-Catenin/cyclinD1 Signaling Pathway. Int J Biol Sci 13:976-984 (2017). Read more (PubMed: 28924379) »
  • Franek M  et al. Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction. J Histochem Cytochem 64:669-686 (2016). Read more (PubMed: 27680669) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (ES cells)
Gel Running Conditions
Reduced Denaturing (10 % gel)
Loading amount
10 µg
Specification
ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Christel Krueger

Verified customer

Submitted Aug 12 2016

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