• Product name

  • Description

    Rabbit polyclonal to CARM1
  • Host species

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human CARM1 aa 550-585 (C terminal).
    Database link: Q86X55

  • Positive control

    • Human breast adenocarcinoma. Human prostate adenocarcinoma.


Our Abpromise guarantee covers the use of ab84370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: 1/100 - 1/500.

    Antigen exposure is recommended.
    Antigen exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
    • Tissue specificity

      Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
    • Sequence similarities

      Belongs to the protein arginine N-methyltransferase family.
    • Post-translational

      Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing.
      Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
    • Cellular localization

      Nucleus. Cytoplasm. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • carm1 antibody
      • CARM1_HUMAN antibody
      • Coactivator associated arginine methyltransferase 1 antibody
      • Coactivator-associated arginine methyltransferase 1 antibody
      • Histone arginine methyltransferase CARM 1 antibody
      • Histone arginine methyltransferase CARM1 antibody
      • Histone-arginine methyltransferase CARM1 antibody
      • PRMT 4 antibody
      • PRMT4 antibody
      • Protein arginine methyltransferase antibody
      • Protein arginine N methyltransferase 4 antibody
      • Protein arginine N-methyltransferase 4 antibody
      see all


    • ab84370 at 1/100, staining human CARM1 in a section of human breast adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    • ab84370 at 1/500, staining human CARM1 in a section of human prostate adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).


    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Western blot
    Human Cell lysate - whole cell (u2os)
    Loading amount
    80 µg
    Gel Running Conditions
    Reduced Denaturing (4-20)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jun 10 2011

    Immunocytochemistry/ Immunofluorescence
    Human Cultured Cells (u2os)
    Yes - triton
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 14 2010

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