Anti-Carma 1/CARD11 antibody (ab113409)

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Overview

  • Product name

    Anti-Carma 1/CARD11 antibody
    See all Carma 1/CARD11 primary antibodies

  • Description

    Rabbit polyclonal to Carma 1/CARD11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow

  • Immunogen

    Recombinant full length protein corresponding to Human Carma 1/CARD11.

  • Positive control

    • Human Liver and Brain cerebellum tissues; A431 and Raji whole cell lysates. The pattern of staining with this antibody is similar to that observed with ab189167 and ab115471 in that the reagent stains macrophages, mast cells, subsets of lymphocytes and plasma cells in various organs across the body. It also stains adrenal cortex, heart, kidney, small intestine epithelium, skeletal muscle, and pancreatic exocrine epithelium and Kupffer cells in the liver

  • General notes

    Previously labelled as CARD11.

Properties

Applications

Our Abpromise guarantee covers the use of ab113409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 133 kDa.
IHC-P 1/100.

Antigen retrieval conditions: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minute

Target

  • Function

    Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.

  • Tissue specificity

    Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.

  • Sequence similarities

    Contains 1 CARD domain.
    Contains 1 guanylate kinase-like domain.
    Contains 1 PDZ (DHR) domain.

  • Post-translational
    modifications

    Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.

  • Cellular localization

    Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

  • Database links

    • Alternative names

      • 0610008L17Rik antibody
      • 2410011D02Rik antibody
      • Bcl10 interacting maguk protein 3 antibody
      • BIMP 3 antibody
      • BIMP3 antibody
      • CAR11_HUMAN antibody
      • CARD 11 antibody
      • CARD containing MAGUK protein 3 antibody
      • Card maguk protein 1 antibody
      • CARD-containing MAGUK protein 1 antibody
      • CARD11 antibody
      • CARD11 protein antibody
      • Carma 1 antibody
      • CARMA1 antibody
      • Caspase recruitment domain containing protein 11 antibody
      • Caspase recruitment domain family member 11 antibody
      • Caspase recruitment domain-containing protein 11 antibody
      • MGC133069 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)

      Immunohistochemistry of Formalin-fixed, Paraffin-embedded Kupffer cells in the sinusoids of the liver labeling Carma 1/CARD11 using ab113409 at 10 µg/ml. Image shows staining of macrophages, mast cells, subsets of lymphocytes and plasma cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)

      Immunohistochemistry of Formalin-fixed, Paraffin-embedded Purkinje cells and fibers in the human cerebellum labeling Carma 1/CARD11 using ab113409 at 10 µg/ml. Image shows staining of macrophages, mast cells, subsets of lymphocytes and plasma cells.

       

    • Western blot - Anti-Carma 1/CARD11 antibody (ab113409)
      Western blot - Anti-Carma 1/CARD11 antibody (ab113409)
      All lanes : Anti-Carma 1/CARD11 antibody (ab113409) at 1/1000 dilution

      Lane 1 : A431 whole cell lysate
      Lane 2 : Raji whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 133 kDa



      5% SDS PAGE

    References

    This product has been referenced in:

    • Watt SA  et al. Novel CARD11 Mutations in Human Cutaneous Squamous Cell Carcinoma Lead to Aberrant NF-?B Regulation. Am J Pathol 185:2354-63 (2015). Read more (PubMed: 26212909) »
    See 1 Publication for this product

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