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    carma-1card11-antibody-ab113409.pdf

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Immunology Adaptive Immunity B Cells Non-CD
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Anti-Carma 1/CARD11 antibody (ab113409)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)
  • Western blot - Anti-Carma 1/CARD11 antibody (ab113409)

Key features and details

  • Rabbit polyclonal to Carma 1/CARD11
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Carma 1/CARD11 antibody
    See all Carma 1/CARD11 primary antibodies
  • Description

    Rabbit polyclonal to Carma 1/CARD11
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human Carma 1/CARD11.

  • Positive control

    • Human Liver and Brain cerebellum tissues; A431 and Raji whole cell lysates. The pattern of staining with this antibody is similar to that observed with ab189167 and ab115471 in that the reagent stains macrophages, mast cells, subsets of lymphocytes and plasma cells in various organs across the body. It also stains adrenal cortex, heart, kidney, small intestine epithelium, skeletal muscle, and pancreatic exocrine epithelium and Kupffer cells in the liver
  • General notes

    Previously labelled as CARD11.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 89% Tris glycine, 10% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Raji membrane extract lysate (ab30126)
    • A-431 membrane extract lysate (ab30134)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab113409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Cow
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 133 kDa.
IHC-P
1/100.

Antigen retrieval conditions: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minute

Notes
WB
1/500 - 1/3000. Predicted molecular weight: 133 kDa.
IHC-P
1/100.

Antigen retrieval conditions: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minute

Target

  • Function

    Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
  • Tissue specificity

    Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 1 guanylate kinase-like domain.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications

    Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
  • Cellular localization

    Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Target information above from: UniProt accession Q9BXL7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 515377 Cow
    • Entrez Gene: 84433 Human
    • Entrez Gene: 108723 Mouse
    • Omim: 607210 Human
    • SwissProt: Q9BXL7 Human
    • SwissProt: Q8CIS0 Mouse
    • Unigene: 648101 Human
    • Unigene: 46187 Mouse
    • Alternative names

      • 0610008L17Rik antibody
      • 2410011D02Rik antibody
      • Bcl10 interacting maguk protein 3 antibody
      • BIMP 3 antibody
      • BIMP3 antibody
      • CAR11_HUMAN antibody
      • CARD 11 antibody
      • CARD containing MAGUK protein 3 antibody
      • Card maguk protein 1 antibody
      • CARD-containing MAGUK protein 1 antibody
      • CARD11 antibody
      • CARD11 protein antibody
      • Carma 1 antibody
      • CARMA1 antibody
      • Caspase recruitment domain containing protein 11 antibody
      • Caspase recruitment domain family member 11 antibody
      • Caspase recruitment domain-containing protein 11 antibody
      • MGC133069 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)

      Immunohistochemistry of Formalin-fixed, Paraffin-embedded Kupffer cells in the sinusoids of the liver labeling Carma 1/CARD11 using ab113409 at 10 µg/ml. Image shows staining of macrophages, mast cells, subsets of lymphocytes and plasma cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)

      Immunohistochemistry of Formalin-fixed, Paraffin-embedded Purkinje cells and fibers in the human cerebellum labeling Carma 1/CARD11 using ab113409 at 10 µg/ml. Image shows staining of macrophages, mast cells, subsets of lymphocytes and plasma cells.

       

    • Western blot - Anti-Carma 1/CARD11 antibody (ab113409)
      Western blot - Anti-Carma 1/CARD11 antibody (ab113409)
      All lanes : Anti-Carma 1/CARD11 antibody (ab113409) at 1/1000 dilution

      Lane 1 : A431 whole cell lysate
      Lane 2 : Raji whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 133 kDa



      5% SDS PAGE

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab113409? Please let us know so that we can cite the reference in this datasheet.

    ab113409 has been referenced in 1 publication.

    • Watt SA  et al. Novel CARD11 Mutations in Human Cutaneous Squamous Cell Carcinoma Lead to Aberrant NF-?B Regulation. Am J Pathol 185:2354-63 (2015). PubMed: 26212909

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