Anti-Carma 1/CARD11 antibody (ab113409)
Key features and details
- Rabbit polyclonal to Carma 1/CARD11
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Carma 1/CARD11 antibody
See all Carma 1/CARD11 primary antibodies -
Description
Rabbit polyclonal to Carma 1/CARD11 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Carma 1/CARD11.
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Positive control
- Human Liver and Brain cerebellum tissues; A431 and Raji whole cell lysates. The pattern of staining with this antibody is similar to that observed with ab189167 and ab115471 in that the reagent stains macrophages, mast cells, subsets of lymphocytes and plasma cells in various organs across the body. It also stains adrenal cortex, heart, kidney, small intestine epithelium, skeletal muscle, and pancreatic exocrine epithelium and Kupffer cells in the liver
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General notes
Previously labelled as CARD11.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89% Tris glycine, 10% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
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| WB |
Human
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| All applications |
Mouse
Cow
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 133 kDa.
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|
| IHC-P |
1/100.
Antigen retrieval conditions: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minute |
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 133 kDa. |
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IHC-P
1/100. Antigen retrieval conditions: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minute |
Target
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Function
Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. -
Tissue specificity
Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsPhosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling. -
Cellular localization
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 515377 Cow
- Entrez Gene: 84433 Human
- Entrez Gene: 108723 Mouse
- Omim: 607210 Human
- SwissProt: Q9BXL7 Human
- SwissProt: Q8CIS0 Mouse
- Unigene: 648101 Human
- Unigene: 46187 Mouse
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Alternative names
- 0610008L17Rik antibody
- 2410011D02Rik antibody
- Bcl10 interacting maguk protein 3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)
Immunohistochemistry of Formalin-fixed, Paraffin-embedded Kupffer cells in the sinusoids of the liver labeling Carma 1/CARD11 using ab113409 at 10 µg/ml. Image shows staining of macrophages, mast cells, subsets of lymphocytes and plasma cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab113409)Immunohistochemistry of Formalin-fixed, Paraffin-embedded Purkinje cells and fibers in the human cerebellum labeling Carma 1/CARD11 using ab113409 at 10 µg/ml. Image shows staining of macrophages, mast cells, subsets of lymphocytes and plasma cells.
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Western blot - Anti-Carma 1/CARD11 antibody (ab113409)All lanes : Anti-Carma 1/CARD11 antibody (ab113409) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 133 kDa
5% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab113409 has been referenced in 1 publication.
- Watt SA et al. Novel CARD11 Mutations in Human Cutaneous Squamous Cell Carcinoma Lead to Aberrant NF-?B Regulation. Am J Pathol 185:2354-63 (2015). PubMed: 26212909