Overview

  • Product name

    Anti-Carma 1/CARD11 antibody
    See all Carma 1/CARD11 primary antibodies
  • Description

    Rabbit polyclonal to Carma 1/CARD11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Carma 1/CARD11 (C terminal).
    Database link: Q9BXL7

  • Positive control

    • Human spleen tissue; mouse spleen cells; mouse thymus cell lysate. The pattern of staining with this antibody is similar to that observed with ab113409 and ab115471 in that the reagent stains macrophages, mast cells, subsets of lymphocytes and plasma cells in various organs across the body. It also stains adrenal cortex, heart, kidney, small intestine epithelium, skeletal muscle, and pancreatic exocrine epithelium and Kupffer cells in the liver.
  • General notes

    Previously labelled as CARD11.

Properties

Applications

Our Abpromise guarantee covers the use of ab189167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 133 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
  • Tissue specificity

    Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 1 guanylate kinase-like domain.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications

    Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
  • Cellular localization

    Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • 0610008L17Rik antibody
    • 2410011D02Rik antibody
    • Bcl10 interacting maguk protein 3 antibody
    • BIMP 3 antibody
    • BIMP3 antibody
    • CAR11_HUMAN antibody
    • CARD 11 antibody
    • CARD containing MAGUK protein 3 antibody
    • Card maguk protein 1 antibody
    • CARD-containing MAGUK protein 1 antibody
    • CARD11 antibody
    • CARD11 protein antibody
    • Carma 1 antibody
    • CARMA1 antibody
    • Caspase recruitment domain containing protein 11 antibody
    • Caspase recruitment domain family member 11 antibody
    • Caspase recruitment domain-containing protein 11 antibody
    • MGC133069 antibody
    see all

Images

  • Lane 1 : Anti-Carma 1/CARD11 antibody (ab189167) at 0.5 µg/ml
    Lane 2 : Anti-Carma 1/CARD11 antibody (ab189167) at 1 µg/ml
    Lane 3 : Anti-Carma 1/CARD11 antibody (ab189167) at 2 µg/ml

    All lanes : Mouse thymus cell lysate

    Predicted band size: 133 kDa

  • Immunofluorescence analysis of mouse spleen cells cells, labeling Carma 1/CARD11 with ab189167 at 20 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue, labeling Carma 1/CARD11 with ab189167 at 5 µg/ml. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References

ab189167 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (lymph node)
Permeabilization
Yes - fix/perm buffer invitrogen
Gating Strategy
Alive single cells
Specification
lymph node
Preparation
Cell harvesting/tissue preparation method: Harvest LN, enzymatic digestion and mechanical dissociation, column lineage + and - , FcR blocking and staining
Sample buffer: MACS buffer
Fixation
Paraformaldehyde

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Verified customer

Submitted Nov 26 2018

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