Key features and details
- Rabbit polyclonal to Carma 1/CARD11
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Carma 1/CARD11 antibody
See all Carma 1/CARD11 primary antibodies
DescriptionRabbit polyclonal to Carma 1/CARD11
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Carma 1/CARD11 (C terminal).
Database link: Q9BXL7
- Human spleen tissue; mouse spleen cells; mouse thymus cell lysate. The pattern of staining with this antibody is similar to that observed with ab113409 and ab115471 in that the reagent stains macrophages, mast cells, subsets of lymphocytes and plasma cells in various organs across the body. It also stains adrenal cortex, heart, kidney, small intestine epithelium, skeletal muscle, and pancreatic exocrine epithelium and Kupffer cells in the liver.
Previously labelled as CARD11.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 133 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Tissue specificityDetected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
Sequence similaritiesContains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain.
modificationsPhosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- 0610008L17Rik antibody
- 2410011D02Rik antibody
- Bcl10 interacting maguk protein 3 antibody
Lane 1 : Anti-Carma 1/CARD11 antibody (ab189167) at 0.5 µg/ml
Lane 2 : Anti-Carma 1/CARD11 antibody (ab189167) at 1 µg/ml
Lane 3 : Anti-Carma 1/CARD11 antibody (ab189167) at 2 µg/ml
All lanes : Mouse thymus cell lysate
Predicted band size: 133 kDa
Immunofluorescence analysis of mouse spleen cells cells, labeling Carma 1/CARD11 with ab189167 at 20 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue, labeling Carma 1/CARD11 with ab189167 at 5 µg/ml. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
ab189167 has not yet been referenced specifically in any publications.