Product nameAnti-Carma 1/CARD11 antibody
See all Carma 1/CARD11 primary antibodies
DescriptionRabbit polyclonal to Carma 1/CARD11
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human Carma 1/CARD11 aa 475-525. The exact sequence is proprietary. NP_115791.3
Database link: Q9BXL7
- WB: Ramos and K562 whole cell lysate. IP: Ramos whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 133 kDa.|
FunctionInvolved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Tissue specificityDetected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
Sequence similaritiesContains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain.
modificationsPhosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- 0610008L17Rik antibody
- 2410011D02Rik antibody
- Bcl10 interacting maguk protein 3 antibody
All lanes : Anti-Carma 1/CARD11 antibody (ab264296) at 0.04 µg/ml
Lane 1 : Ramos whole cell lysate at 50 µg
Lane 2 : Ramos whole cell lysate at 15 µg
Lane 3 : Ramos whole cell lysate at 5 µg
Lane 4 : K562 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 133 kDa
Exposure time: 3 minutes
Carma 1/CARD11 was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab264296 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264296 at 1 µg/ml.
Lane 1: ab264296 IP in Ramos whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264296 has not yet been referenced specifically in any publications.