Key features and details
- Rabbit polyclonal to Carma 1/CARD11
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Carma 1/CARD11 antibody
See all Carma 1/CARD11 primary antibodies
DescriptionRabbit polyclonal to Carma 1/CARD11
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Carma 1/CARD11 aa 575-625. The exact sequence is proprietary. NP_115791.3
Database link: Q9BXL7
- WB: K562 and Ramos whole cell lysate. IP: Ramos whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 133 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionInvolved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Tissue specificityDetected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
Sequence similaritiesContains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain.
modificationsPhosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- 0610008L17Rik antibody
- 2410011D02Rik antibody
- Bcl10 interacting maguk protein 3 antibody
All lanes : Anti-Carma 1/CARD11 antibody (ab264297) at 0.1 µg/ml
Lane 1 : Ramos whole cell lysate at 50 µg
Lane 2 : Ramos whole cell lysate at 15 µg
Lane 3 : Ramos whole cell lysate at 5 µg
Lane 4 : K562 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 133 kDa
Exposure time: 3 minutes
Carma 1/CARD11 was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with at ab264297 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264297 at 1 µg/ml.
Lane 1: ab264297 IP in Ramos whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264297 has not yet been referenced specifically in any publications.