Key features and details
- Goat polyclonal to Carma 1/CARD11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Carma 1/CARD11 antibody
See all Carma 1/CARD11 primary antibodies
DescriptionGoat polyclonal to Carma 1/CARD11
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Dog
GenBank Accession Number – NP_115791. LocusLink ID - 84433 (human). Gene Ontology terms - guanylate kinase activity; protein binding; intracellular signaling cascade; apoptosis; kinesin complex; intracellular; membrane fraction; apoptosis regulator activity; activation of NF-kappaB-inducing kinase.
Previously labelled as CARD11.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab4358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 133 kDa).
An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Tissue specificityDetected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
Sequence similaritiesContains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain.
modificationsPhosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- 0610008L17Rik antibody
- 2410011D02Rik antibody
- Bcl10 interacting maguk protein 3 antibody
Anti-Carma 1/CARD11 antibody (ab4358) at 1 µg/ml + Human Jurkat cells (RIPA buffer, 35µg total protein per lane)
Predicted band size: 133 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ab4358 has not yet been referenced specifically in any publications.