• Product name

    Anti-Carma 1/CARD11 antibody
    See all Carma 1/CARD11 primary antibodies
  • Description

    Rabbit polyclonal to Carma 1/CARD11
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human Carma 1/CARD11 aa 275-325.
    Database link: NP_115791.3

  • Positive control

    • Ramos cell lysate and K562 cell lysate
  • General notes

    Previously labelled as CARD11.



Our Abpromise guarantee covers the use of ab91463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23129749
WB 1/2000 - 1/10000. Predicted molecular weight: 133 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function

    Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
  • Tissue specificity

    Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 1 guanylate kinase-like domain.
    Contains 1 PDZ (DHR) domain.
  • Post-translational

    Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
  • Cellular localization

    Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • 0610008L17Rik antibody
    • 2410011D02Rik antibody
    • Bcl10 interacting maguk protein 3 antibody
    • BIMP 3 antibody
    • BIMP3 antibody
    • CAR11_HUMAN antibody
    • CARD 11 antibody
    • CARD containing MAGUK protein 3 antibody
    • Card maguk protein 1 antibody
    • CARD-containing MAGUK protein 1 antibody
    • CARD11 antibody
    • CARD11 protein antibody
    • Carma 1 antibody
    • CARMA1 antibody
    • Caspase recruitment domain containing protein 11 antibody
    • Caspase recruitment domain family member 11 antibody
    • Caspase recruitment domain-containing protein 11 antibody
    • MGC133069 antibody
    see all


  • All lanes : Anti-Carma 1/CARD11 antibody (ab91463) at 0.1 µg/ml

    Lane 1 : Ramos whole cell lysate at 50 µg
    Lane 2 : Ramos whole cell lysate at 15 µg
    Lane 3 : Ramos whole cell lysate at 5 µg
    Lane 4 : K562 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 133 kDa

    Exposure time: 3 minutes
  • Sample: whole cell lysate from Ramos cells (1 mg for IP, 20% of IP loaded) .
    Antibody: ab91463 at 1 µg/ml for WB and at 10 µg/mg lysate for IP.
    Detection: chemiluminescence with exposure times of 10 seconds.


This product has been referenced in:

  • Snow AL  et al. Congenital B cell lymphocytosis explained by novel germline CARD11 mutations. J Exp Med 209:2247-61 (2012). ICC/IF ; Human . Read more (PubMed: 23129749) »
See 1 Publication for this product

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