Product nameAnti-Carma 1/CARD11 antibody
See all Carma 1/CARD11 primary antibodies
DescriptionRabbit polyclonal to Carma 1/CARD11
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
Synthetic peptide corresponding to Human Carma 1/CARD11 aa 275-325.
Database link: NP_115791.3
- Ramos cell lysate and K562 cell lysate
Previously labelled as CARD11.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23129749|
|WB||1/2000 - 1/10000. Predicted molecular weight: 133 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionInvolved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Tissue specificityDetected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
Sequence similaritiesContains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain.
modificationsPhosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- 0610008L17Rik antibody
- 2410011D02Rik antibody
- Bcl10 interacting maguk protein 3 antibody
All lanes : Anti-Carma 1/CARD11 antibody (ab91463) at 0.1 µg/ml
Lane 1 : Ramos whole cell lysate at 50 µg
Lane 2 : Ramos whole cell lysate at 15 µg
Lane 3 : Ramos whole cell lysate at 5 µg
Lane 4 : K562 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 133 kDa
Exposure time: 3 minutes
Sample: whole cell lysate from Ramos cells (1 mg for IP, 20% of IP loaded) .
Antibody: ab91463 at 1 µg/ml for WB and at 10 µg/mg lysate for IP.
Detection: chemiluminescence with exposure times of 10 seconds.