Overview

  • Product name

  • Description

    Rabbit polyclonal to Carma 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Carma 3 (C terminal). Synthetic peptide corresponding to 14 amino acids at the C terminus of human Carma 3.
    Database link: AAK26165
    (Peptide available as ab39737)

  • Positive control

    • EL4 cell lysate can be used as a positive control.
  • General notes

    Protein previously labeled as CARD10.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 116 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Activates NF-kappa-B via BCL10 and IKK.
  • Tissue specificity

    Detected in adult heart, kidney and liver; lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney.
  • Sequence similarities

    Contains 1 CARD domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bcl10 binding protein and activator of NFKB antibody
    • BIMP1 antibody
    • CAR10_HUMAN antibody
    • CARD 10 antibody
    • CARD containing MAGUK 3 protein antibody
    • CARD-containing MAGUK 3 antibody
    • CARD-containing MAGUK protein 3 antibody
    • CARD10 antibody
    • Carma 3 antibody
    • CARMA3 antibody
    • Caspase Recruitment Domain family member 10 antibody
    • Caspase recruitment domain-containing protein 10 antibody
    see all

Images

  • Anti-Carma 3 antibody (ab36839) at 5 µg/ml + A20 cell lysate

    Predicted band size: 116 kDa

  • Immunofluorescence of Carma 3 in EL4 cells using ab36839 at 20 µg/ml.

References

This product has been referenced in:

  • Zhang X  et al. The circINTS4/miR-146b/CARMA3 axis promotes tumorigenesis in bladder cancer. Cancer Gene Ther N/A:N/A (2019). Read more (PubMed: 30723269) »
  • Lin J  et al. MicroRNA-181b inhibits thrombin-mediated endothelial activation and arterial thrombosis by targeting caspase recruitment domain family member 10. FASEB J 30:3216-26 (2016). Read more (PubMed: 27297585) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

Thank you for contacting us regarding this. I could not find any historical data showing that we had offered an anti-Card10 or anti-CARM3 raised in sheep. I hope this information is helpful. Please let me know if have any questions.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate Buffer pH 6
Permeabilization
No
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Buffered Formalin

Abcam user community

Verified customer

Submitted Sep 30 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (muscle)
Permeabilization
No
Specification
muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Sep 25 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH 6
Permeabilization
No
Specification
pancreatic tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin

Abcam user community

Verified customer

Submitted Sep 09 2015

Answer

Thank you for your enquiry. I have enquired at the originator of the product and they have confirmed that the immunogen for this antibody has no homology with CARD11 and a BLAST search does not yield proteins that large, so cross-reaction with linear epitopes of other proteins is highly unlikely. We typically screen a panel of tissues and cell lines and deem any that are in the approximate size range based on the predicted MW (116kDa for CARD10) and the banding patterns given to be positive. The smaller size band seen on our data sheet could be a splicing variant, cleaved fragment, or some other modification. The markers we used are dyed and can run larger making the proteins appear smaller. Unfortunately, we do not have plans to develop another antibody at this time. I hope the information above will be useful to you. Should you require any further information, please do not hesitate to contact me.

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