Key features and details
- Rabbit polyclonal to CARS
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CARS antibody
See all CARS primary antibodies
DescriptionRabbit polyclonal to CARS
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken, Cynomolgus monkey
Recombinant fragment corresponding to Human CARS aa 201-500.
TLGCDVTDNSIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVSEYVPEIVN FVQKIVDNGYGYVSNGSVYFDTAKFASSEKHSYGKLVPEAVGDQKALQEG EGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSA MAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHL TIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTME SALQYEKFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTA
Database link: P49589
- WB: PC-3 whole cell lysate. Mouse lung and kidney tissue lysate. IHC-P: Human pancreas tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/2000 - 1/5000.|
Involvement in diseaseNote=A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
- Information by UniProt
- Cysteine tRNA ligase antibody
- Cars antibody
- CysRS antibody
All lanes : Anti-CARS antibody (ab235536) at 1/2000 dilution
Lane 1 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse kidney tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Paraffin-embedded human pancreas tissue stained for CARS with ab235536 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235536 has not yet been referenced specifically in any publications.