Anti-CARS antibody [CL2304] (ab242364)

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Overview

  • Product name

    Anti-CARS antibody [CL2304]
    See all CARS primary antibodies

  • Description

    Mouse monoclonal [CL2304] to CARS

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human CARS aa 523-658.
    Sequence:

    SQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVG GPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNIL PELGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVE


    Database link: P49589

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    Binds to an epitope located within the peptide sequence EDSSLGFPVG as determined by overlapping synthetic peptides.

  • Positive control

    • IHC-P: Human Placenta, skin, breast cancer, colon and kidney tissue. WB: U-251 MG and HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab242364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa.
IHC-P 1/5000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Involvement in disease

    Note=A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.

  • Sequence similarities

    Belongs to the class-I aminoacyl-tRNA synthetase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cysteine tRNA ligase antibody
      • Cars antibody
      • CysRS antibody
      • Cysteine--tRNA ligase antibody
      • Cysteinyl tRNA synthetase, cytoplasmic antibody
      • Cysteinyl-tRNA synthetase antibody
      • cytoplasmic antibody
      • MGC:11246 antibody
      • SYCC_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2304] (ab242364)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2304] (ab242364)

      Paraffin-embedded human kidney tissue stained for CARS using ab242364 at 1/5000 dilution in immunohistochemical analysis. 

    • Western blot - Anti-CARS antibody [CL2304] (ab242364)
      Western blot - Anti-CARS antibody [CL2304] (ab242364)
      All lanes : Anti-CARS antibody [CL2304] (ab242364) at 1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate cytoplasmic fraction
      Lane 2 : HeLa membrane fraction
      Lane 3 : HeLa nuclear fraction
      Lane 4 : HeLa chromatin fraction
      Lane 5 : HeLa cytoskeleton fraction

      Predicted band size: 85 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2304] (ab242364)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2304] (ab242364)

      Paraffin-embedded human colon tissue stained for CARS using ab242364 at 1/5000 dilution in immunohistochemical analysis. 

    • Western blot - Anti-CARS antibody [CL2304] (ab242364)
      Western blot - Anti-CARS antibody [CL2304] (ab242364)
      Anti-CARS antibody [CL2304] (ab242364) at 1 µg/ml + U-251 MG (human brain glioma cell line) whole cell lysate

      Predicted band size: 85 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2304] (ab242364)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2304] (ab242364)

      Paraffin-embedded human breast cancer tissue stained for CARS using ab242364 at 1/5000 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2304] (ab242364)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2304] (ab242364)

      Paraffin-embedded human skin tissue stained for CARS using ab242364 at 1/5000 dilution in immunohistochemical analysis. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2304] (ab242364)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2304] (ab242364)

      Paraffin-embedded human placenta tissue stained for CARS using ab242364 at 1/5000 dilution in immunohistochemical analysis.

    References

    ab242364 has not yet been referenced specifically in any publications.

    Publishing research using ab242364? Please let us know so that we can cite the reference in this datasheet.

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