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Rabbit monoclonal [EPR7121] to CARS
, Flow Cyt
, ICC/IFmore detailsUnsuitable for:
A synthetic peptide corresponding to residues in human CARS (P49589)
- 293T cell lysate
HeLa cell lysate
U87-MG cell lysate
BxPC-3 cell lysate
MCF-7 cell lysate
human breast carcinoma tissue
A549 cell lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/10000 - 1/50000. Predicted molecular weight: 85 kDa.
||1/50 - 1/100. Antigen retrieval step is recommended.
||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
||1/50 - 1/100.
Is unsuitable for IP.
Involvement in disease
Note=A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.
Belongs to the class-I aminoacyl-tRNA synthetase family.
Information by UniProt
- Cysteine tRNA ligase antibody
- Cars antibody
- CysRS antibody
Western blot - Anti-CARS antibody [EPR7121] (ab126714)
All lanes : Anti-CARS antibody [EPR7121] (ab126714) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : BxPC-3 cell lysate
Lane 5 : MCF-7 cell lysate
Lane 6 : A549 cell lysate
Predicted band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [EPR7121] (ab126714)
ab126714, at 1/50 dilution, stains CARS in paraffin-embedded human breast carcinoma tissue by immunohistochemistry.
Other - Anti-CARS antibody [EPR7121] (ab126714)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"