Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7121] to CARS
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-CARS antibody [EPR7121]
See all CARS primary antibodies
DescriptionRabbit monoclonal [EPR7121] to CARS
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
A synthetic peptide corresponding to residues in human CARS (P49589)
- 293T cell lysate HeLa whole cell lysate (ab150035) U87-MG cell lysate BxPC-3 cell lysate MCF-7 cell lysate human breast carcinoma tissue A549 cell lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 85 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval step is recommended.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/100.|
Involvement in diseaseNote=A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
- Information by UniProt
- Cysteine tRNA ligase antibody
- Cars antibody
- CysRS antibody
All lanes : Anti-CARS antibody [EPR7121] (ab126714) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : BxPC-3 cell lysate
Lane 5 : MCF-7 cell lysate
Lane 6 : A549 cell lysate
Predicted band size: 85 kDa
ab126714, at 1/50 dilution, stains CARS in paraffin-embedded human breast carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab126714 has not yet been referenced specifically in any publications.