Key features and details
- Rabbit polyclonal to CARS2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CARS2 antibody
DescriptionRabbit polyclonal to CARS2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- ICC/IF: A431 cells. WB: U87 MG cell lysate. IHC-P: Human adrenal gland, endometrium, uterine cercix, cerebral cortex and testis tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 54 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceCARS2 (Cysteinyl tRNA synthetase 2) belongs to the class-I aminoacyl-tRNA synthetase family. Aminoacyl tRNA synthetases catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two step reaction.
Cellular localizationMitochondrion matrix.
- CARS 2 antibody
- CysRS antibody
- Cysteine tRNA ligase 2 antibody
PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling CARS2 using ab254863 at 4µg/ml (green) in ICC/IF.
Anti-CARS2 antibody (ab254863) at 0.4 µg/ml + U87 MG cell lysate
Predicted band size: 54 kDa
Paraffin-embedded human uterine cervix tissue stained for CARS2 using ab254863 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for CARS2 using ab254863 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for CARS2 using ab254863 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral cortex tissue stained for CARS2 using ab254863 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrium tissue stained for CARS2 using ab254863 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254863 has not yet been referenced specifically in any publications.