Key features and details
- Rabbit polyclonal to CASC4 - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CASC4 antibody - C-terminal
See all CASC4 primary antibodies
DescriptionRabbit polyclonal to CASC4 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human CASC4 aa 336-365 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q6P4E1
- IHC-P: Human prostate and brain cortex tissue. WB: Neuro-2a cell lysate. Flow Cytometry: Neuro-2a cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.|
Sequence similaritiesBelongs to the GOLM1/CASC4 family.
- Information by UniProt
- Cancer susceptibility candidate 4 antibody
- Cancer susceptibility candidate gene 4 protein antibody
- CASC4 antibody
Anti-CASC4 antibody - C-terminal (ab230522) at 1/1000 dilution + Neuro-2a (Mouse neuroblastoma cell line) cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 48 kDa
Formalin-fixed, paraffin-embedded human brain cortex tissue stained for CASC4 with ab230522 at a 1:100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human prostate tissue stained for CASC4 with ab230522 at a 1:100 dilution in immunohistochemical analysis.
Flow Cytometry analysis of Neuro-2a (Mouse neuroblastoma cell line) cells, labeling CASC4 with ab230522 at a 1/10 dilution (right side) compared to a negative control of (left side). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230522 has not yet been referenced specifically in any publications.