Overview

  • Product name

    Anti-CASC4 antibody - C-terminal
    See all CASC4 primary antibodies
  • Description

    Rabbit polyclonal to CASC4 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CASC4 aa 336-365 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q6P4E1

  • Positive control

    • IHC-P: Human prostate and brain cortex tissue. WB: Neuro-2a cell lysate. Flow Cytometry: Neuro-2a cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 48 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

Target

Images

  • Anti-CASC4 antibody - C-terminal (ab230522) at 1/1000 dilution + Neuro-2a (Mouse neuroblastoma cell line) cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 48 kDa

  • Formalin-fixed, paraffin-embedded human brain cortex tissue stained for CASC4 with ab230522 at a 1:100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for CASC4 with ab230522 at a 1:100 dilution in immunohistochemical analysis.

  • Flow Cytometry analysis of Neuro-2a (Mouse neuroblastoma cell line) cells, labeling CASC4 with ab230522 at a 1/10 dilution (right side) compared to a negative control of (left side). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

References

ab230522 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230522.
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