Anti-CASC5/KNL1 antibody (ab70537)
Key features and details
- Rabbit polyclonal to CASC5/KNL1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CASC5/KNL1 antibody
See all CASC5/KNL1 primary antibodies -
Description
Rabbit polyclonal to CASC5/KNL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human CASC5/KNL1 aa 300-400. Synthetic peptide corresponding to Human CASC5/KNL1. Database link: NP_733468.2
Database link: Q8NG31 -
Positive control
- Whole cell lysate from HeLa or 293T cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately >300 kDa (predicted molecular weight: 265 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately >300 kDa (predicted molecular weight: 265 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist. -
Cellular localization
Acrosome. Nucleus. -
Database links
- Entrez Gene: 57082 Human
- Omim: 609173 Human
- SwissProt: Q8NG31 Human
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Alternative names
- Protein D40/AF15q14 antibody
- AF15Q14 antibody
- ALL1 fused gene from chromosome 15q14 antibody
see all
Images
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All lanes : Anti-CASC5/KNL1 antibody (ab70537) at 1/5000 dilution
Lane 1 : Whole cell lysate from 293T cells at 50 µg
Lane 2 : Whole cell lysate from 293T cells at 15 µg
Developed using the ECL technique.
Predicted band size: 265 kDa
Observed band size: >300 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70537 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent blot, 20% of the precipitate was loaded per lane, and ab70537 was used at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab70537 has been referenced in 12 publications.
- Germanova TE et al. Subcellular Euclidean distance measurements with multicolor fluorescence localization imaging in cultured cells. STAR Protoc 2:100774 (2021). PubMed: 34841272
- Cordeiro MH et al. Kinetochore phosphatases suppress autonomous Polo-like kinase 1 activity to control the mitotic checkpoint. J Cell Biol 219:N/A (2020). PubMed: 33125045
- Zhou C et al. The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I. Sci Adv 6:eaax3969 (2020). PubMed: 32284991
- Roscioli E et al. Ensemble-Level Organization of Human Kinetochores and Evidence for Distinct Tension and Attachment Sensors. Cell Rep 31:107535 (2020). PubMed: 32348762
- Vallardi G et al. Division of labour between PP2A-B56 isoforms at the centromere and kinetochore. Elife 8:N/A (2019). PubMed: 30829571