Overview

  • Product name
    Anti-Casein antibody [F20.14]
    See all Casein primary antibodies
  • Description
    Mouse monoclonal [F20.14] to Casein
  • Host species
    Mouse
  • Specificity

    This antibody reacts with beta-casein. The reactivity with alpha-casein has not been tested. 

    This antibody does not react with alpha lactalbumin. This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.

     

  • Tested applications
    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A/G purified
  • Clonality
    Monoclonal
  • Clone number
    F20.14
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa).
IHC-Fr Use at an assay dependent concentration. Fix with acetone.

Target

  • Function
    Important role in the capacity of milk to transport calcium phosphate.
    Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors.
  • Tissue specificity
    Mammary gland specific. Secreted in milk.
  • Sequence similarities
    Belongs to the alpha-casein family.
  • Post-translational
    modifications
    Not glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha casein antibody
    • alpha-S1-casein antibody
    • CASA antibody
    • CASA1_HUMAN antibody
    • Casein alpha s1 antibody
    • casein, alpha antibody
    • casein, alpha-S1, included antibody
    • Casoxin-D antibody
    • CSN1 antibody
    • CSN1S1 antibody
    • Csna antibody
    • MGC149368 antibody
    see all

Images

  • ab47972 staining Casein in Human lactating breast tissue biopsy by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1% normal serum containing 0.05% Tween for 30 minutes at room temperature. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour. A biotin-conjugated Donkey anti-Mouse IgG was used as the secondary antibody, diluted 1/250.

    See Abreview

References

This product has been referenced in:
  • Bonuccelli G  et al. The milk protein a-casein functions as a tumor suppressor via activation of STAT1 signaling, effectively preventing breast cancer tumor growth and metastasis. Cell Cycle 11:3972-82 (2012). Read more (PubMed: 23047602) »
  • Burchell J  et al. Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and alpha-lactalbumin. Hybridoma 4:341-50 (1985). Read more (PubMed: 3905581) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ortholog, and to my knowledge, in most experimental settings the bovine protein is detected in human breast milk (e.g. PMID8163788). If this is of interest for the customer, I am happy to let you know that we have two antibodies against the bovine beta-lactoglobulin in our catalog (ab112893, ab112894). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112893). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112894). 2) Alpha-lactalbumin: Again, we have antibodies against the bovine protein, but also one antibody against the human protein which are guaranteed to work in ELISA (ab112972, ab112973, ab35288). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112972). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112973). Click here (or use the following: https://www.abcam.com/index.html?datasheet=35288). 3) Casein: We only have one antibody against the human Casein, suitable for ELISA (ab91167). Click here (or use the following: https://www.abcam.com/index.html?datasheet=91167). 4) Lactoferrin: We have a number of antibodies available against the human lactoferrin some of which are not only guaranteed to work in a conventional ELISA, but also in Sandwich ELISA (sELISA), for example ab10110 or ab15811. Click here (or use the following: https://www.abcam.com/index.html?datasheet=10110). Click here (or use the following: https://www.abcam.com/index.html?datasheet=15811). Please find attached our ELISA protocols. If the customer is interested in matched antibody pairs for Sandwich ELISA, or antibodies against these targets from other species than human, I would be pleased to assist in finding the most suitable ones. Please be reassured that all antibodies are covered by our Abpromise: We are happy to offer technical support, and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. All tested species and applications are specified on our datasheets, which are updated as soon as any new information is brought to our attention. If the customer decides to go ahead and test these products in an untested species or application, please contact our Scientific Support team prior to the purchase as they might be eligible for our testing discount program. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human lactating breast biopsy)
Specification
Human lactating breast biopsy
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Normal serum containing 0.05% Tween as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

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Verified customer

Submitted Sep 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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