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Dear Sir/Madam The customer will set ELISA application to detect the above 4 protein from human breast milk. 1. Beta-lactoglobulin : AB8226, AB8227 2. Alpha-lactalbumin : AB20212,AB112972, AB112973 3. Casein : AB47972 4. Lactoferrin : AB108882 Please kindly recommend technical or protocol and ab items No. suitable.
Asked on Dec 09 2011
Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ortholog, and to my knowledge, in most experimental settings the bovine protein is detected in human breast milk (e.g. PMID8163788). If this is of interest for the customer, I am happy to let you know that we have two antibodies against the bovine beta-lactoglobulin in our catalog (ab112893, ab112894). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112893). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112894). 2) Alpha-lactalbumin: Again, we have antibodies against the bovine protein, but also one antibody against the human protein which are guaranteed to work in ELISA (ab112972, ab112973, ab35288). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112972). Click here (or use the following: https://www.abcam.com/index.html?datasheet=112973). Click here (or use the following: https://www.abcam.com/index.html?datasheet=35288). 3) Casein: We only have one antibody against the human Casein, suitable for ELISA (ab91167). Click here (or use the following: https://www.abcam.com/index.html?datasheet=91167). 4) Lactoferrin: We have a number of antibodies available against the human lactoferrin some of which are not only guaranteed to work in a conventional ELISA, but also in Sandwich ELISA (sELISA), for example ab10110 or ab15811. Click here (or use the following: https://www.abcam.com/index.html?datasheet=10110). Click here (or use the following: https://www.abcam.com/index.html?datasheet=15811). Please find attached our ELISA protocols. If the customer is interested in matched antibody pairs for Sandwich ELISA, or antibodies against these targets from other species than human, I would be pleased to assist in finding the most suitable ones. Please be reassured that all antibodies are covered by our Abpromise: We are happy to offer technical support, and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. All tested species and applications are specified on our datasheets, which are updated as soon as any new information is brought to our attention. If the customer decides to go ahead and test these products in an untested species or application, please contact our Scientific Support team prior to the purchase as they might be eligible for our testing discount program. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Dec 09 2011