Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free (ab223144)

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Overview

  • Product name

    Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free
    See all Casein Kinase 1 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR19824] to Casein Kinase 1 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Casein Kinase 1 alpha aa 50-150. The exact sequence is proprietary.
    Database link: P48729

  • Positive control

    • WB: HEK-293T, HeLa, MCF7 and NIH/3T3 whole cell lysates; Human fetal brain, fetal kidney, fetal spleen and skin lysates; Mouse and rat brain, kidney, spleen and skin lysates. IP: Human skin lysate.

  • General notes

    Ab223144 is the carrier-free version of ab206652. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab223144 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab223144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.

  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Casein kinase 1 alpha 1 antibody
    • Casein kinase I isoform alpha antibody
    • CK1 antibody
    • CK1A antibody
    • CKI alpha antibody
    • CKI-alpha antibody
    • CKIa antibody
    • Clock regulator kinase antibody
    • Csnk1a1 antibody
    • Down regulated in lung cancer antibody
    • HLCDGP1 antibody
    • KC1A_HUMAN antibody
    • PRO2975 antibody
    see all

Images

  • Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free (ab223144)
    Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free (ab223144)

    Casein Kinase 1 alpha was immunoprecipitated from 0.35 mg of Human skin lysate with ab206652 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206652 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human skin lysate, 10µg (Input).

    Lane 2: ab206652 IP in Human skin lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206652 in Human skin lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206652).

  • Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free (ab223144)
    Western blot - Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free (ab223144)
    All lanes : Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HEK-293T Casein Kinase 1 alpha KO whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 39 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206652).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The lysates were kindly provided by our collaborator Dr. Yi Rao, Peking University.

References

ab223144 has not yet been referenced specifically in any publications.

Publishing research using ab223144? Please let us know so that we can cite the reference in this datasheet.

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