Recombinant Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free (ab223144)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19824] to Casein Kinase 1 alpha - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free
See all Casein Kinase 1 alpha primary antibodies -
Description
Rabbit monoclonal [EPR19824] to Casein Kinase 1 alpha - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Casein Kinase 1 alpha aa 50-150. The exact sequence is proprietary.
Database link: P48729 -
Positive control
- WB: HEK-293T, HeLa, MCF7 and NIH/3T3 whole cell lysates; Human fetal brain, fetal kidney, fetal spleen and skin lysates; Mouse and rat brain, kidney, spleen and skin lysates. IP: Human skin lysate.
-
General notes
Ab223144 is the carrier-free version of ab206652. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab223144 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19824 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab223144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa). | |
IP | Use at an assay dependent concentration. |
Target
-
Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. -
Sequence similarities
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. - Information by UniProt
-
Database links
- Entrez Gene: 1452 Human
- Entrez Gene: 93687 Mouse
- Entrez Gene: 113927 Rat
- Omim: 600505 Human
- SwissProt: P48729 Human
- SwissProt: Q8BK63 Mouse
- SwissProt: P97633 Rat
- Unigene: 529862 Human
see all -
Alternative names
- Casein kinase 1 alpha 1 antibody
- Casein kinase I isoform alpha antibody
- CK1 antibody
see all
Images
-
Immunoprecipitation - Anti-Casein Kinase 1 alpha antibody [EPR19824] - BSA and Azide free (ab223144)
Casein Kinase 1 alpha was immunoprecipitated from 0.35 mg of Human skin lysate with ab206652 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206652 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human skin lysate, 10µg (Input).
Lane 2: ab206652 IP in Human skin lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206652 in Human skin lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206652).
-
All lanes : Anti-Casein Kinase 1 alpha antibody [EPR19824] (ab206652) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T Casein Kinase 1 alpha KO whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206652).
Blocking/Dilution buffer: 5% NFDM/TBST.
The lysates were kindly provided by our collaborator Dr. Yi Rao, Peking University.
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab223144 has not yet been referenced specifically in any publications.