Overview

  • Product name

    Anti-Casein Kinase 1 alpha (phospho T321) antibody
    See all Casein Kinase 1 alpha primary antibodies
  • Description

    Rabbit polyclonal to Casein Kinase 1 alpha (phospho T321)
  • Host species

    Rabbit
  • Specificity

    Detects Casein Kinase I alpha only when phosphorylated at threonine 321.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Casein Kinase 1 alpha aa 319-323 (phospho T321).
    Sequence:

    A-Q-Tp-P-T

  • Positive control

    • HuvEc cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA 1/10000.

Target

  • Function

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Casein kinase 1 alpha 1 antibody
    • Casein kinase I isoform alpha antibody
    • CK1 antibody
    • CK1A antibody
    • CKI alpha antibody
    • CKI-alpha antibody
    • CKIa antibody
    • Clock regulator kinase antibody
    • Csnk1a1 antibody
    • Down regulated in lung cancer antibody
    • HLCDGP1 antibody
    • KC1A_HUMAN antibody
    • PRO2975 antibody
    see all

Images

  • All lanes : Anti-Casein Kinase 1 alpha (phospho T321) antibody (ab63559) at 1/500 dilution

    Lane 1 : extracts from HuvEc cells
    Lane 2 : extracts from HuvEc cells with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

References

ab63559 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab63559.
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