Key features and details
- Rabbit polyclonal to Casein Kinase 1 alpha (phospho T321)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Casein Kinase 1 alpha (phospho T321) antibody
See all Casein Kinase 1 alpha primary antibodies
DescriptionRabbit polyclonal to Casein Kinase 1 alpha (phospho T321)
SpecificityDetects Casein Kinase I alpha only when phosphorylated at threonine 321.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- HuvEc cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab63559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
- Information by UniProt
- Casein kinase 1 alpha 1 antibody
- Casein kinase I isoform alpha antibody
- CK1 antibody
ab63559 has not yet been referenced specifically in any publications.