Key features and details
- Goat polyclonal to Casein Kinase 1 delta/CSNK1D
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Casein Kinase 1 delta/CSNK1D antibody
See all Casein Kinase 1 delta/CSNK1D primary antibodies
DescriptionGoat polyclonal to Casein Kinase 1 delta/CSNK1D
This antibody is expected to recognise both reported isoforms of Casein Kinase 1 delta/CSNK1D.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee, Macaque monkey
- WB: HEK 293 cell lysate. ICC/UF U251 cells.
Previously labelled as Casein Kinase 1 delta.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab48031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa).
1 hour primary incubation is recommended for this product.
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.
Tissue specificityExpressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.
Involvement in diseaseDefects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated on serine and threonine residues.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Casein Kinase 1 delta antibody
- Casein kinase I delta isoform antibody
- Casein kinase I isoform delta antibody
Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031) at 0.3 µg/ml + HEK 293 cell lysate in RIPA buffer at 35 µg
Predicted band size: 47 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected using chemiluminescence.
Immunocytochemistry/Immunofluorescence analysis of U251 cells labelling Casein Kinase 1 delta/CSNK1D with ab48031 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
ab48031 has been referenced in 2 publications.
- Zhou A et al. Nuclear GSK3ß promotes tumorigenesis by phosphorylating KDM1A and inducing its deubiquitylation by USP22. Nat Cell Biol 18:954-966 (2016). PubMed: 27501329
- Zyss D et al. Casein kinase I delta controls centrosome positioning during T cell activation. J Cell Biol 195:781-97 (2011). PubMed: 22123863