Overview

  • Product name

    Anti-Casein Kinase 1 delta/CSNK1D antibody
    See all Casein Kinase 1 delta/CSNK1D primary antibodies
  • Description

    Goat polyclonal to Casein Kinase 1 delta/CSNK1D
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise both reported isoforms of Casein Kinase 1 delta/CSNK1D.

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human Casein Kinase 1 delta/CSNK1D aa 310-324 (internal sequence).
    Sequence:

    DREERLRHSRNPATR


    Database link: P48730

  • Positive control

    • WB: HEK 293 cell lysate. ICC/UF U251 cells.
  • General notes

    Previously labelled as Casein Kinase 1 delta. 

Properties

Applications

Our Abpromise guarantee covers the use of ab48031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.
  • Tissue specificity

    Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.
  • Involvement in disease

    Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated on serine and threonine residues.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Casein Kinase 1 delta antibody
    • Casein kinase I delta isoform antibody
    • Casein kinase I isoform delta antibody
    • CKI delta antibody
    • CKI-delta antibody
    • CKId antibody
    • CKIdelta antibody
    • CSNK 1D antibody
    • CSNK1D antibody
    • HCKID antibody
    • KC1D_HUMAN antibody
    • Protein kinase CK1 delta antibody
    see all

Images

  • Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031) at 0.3 µg/ml + HEK 293 cell lysate in RIPA buffer at 35 µg

    Predicted band size: 47 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected using chemiluminescence.

  • Immunocytochemistry/Immunofluorescence analysis of U251 cells labelling Casein Kinase 1 delta/CSNK1D with ab48031 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.

    Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).

References

This product has been referenced in:

  • Zhou A  et al. Nuclear GSK3ß promotes tumorigenesis by phosphorylating KDM1A and inducing its deubiquitylation by USP22. Nat Cell Biol 18:954-966 (2016). Read more (PubMed: 27501329) »
  • Zyss D  et al. Casein kinase I delta controls centrosome positioning during T cell activation. J Cell Biol 195:781-97 (2011). Read more (PubMed: 22123863) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293)
Gel Running Conditions
Non-reduced Denaturing (10)
Loading amount
20 µg
Treatment
DMSO, 24 hours
Specification
HEK 293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

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Verified customer

Submitted Nov 07 2011

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