Key features and details
- Rabbit polyclonal to Casein Kinase 1 gamma 2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Casein Kinase 1 gamma 2 antibody
See all Casein Kinase 1 gamma 2 primary antibodies
DescriptionRabbit polyclonal to Casein Kinase 1 gamma 2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse gonadal tissue; HeLa whole cell lysate. IHC-P: Human testis tissue and human breast cancer tissue. IP: HeLa whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionSerine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.
Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- Casein kinase 1, gamma 2 antibody
- Casein kinase I isoform gamma 2 antibody
- Casein kinase I isoform gamma-2 antibody
All lanes : Anti-Casein Kinase 1 gamma 2 antibody (ab230045) at 1/1000 dilution
Lane 1 : Mouse gonadal tissue lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Casein Kinase 1 gamma 2 was immunoprecipitated from 500 µg HeLa epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg of ab230045. Western blot was performed from the immunoprecipitate using ab230045 at 1/1000 dilution.
Lane 1: 1 µg of Rabbit monoclonal IgG instead of ab230045 in HeLa whole cell lysate.
Lane 2: ab230045 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (input).
For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used the secondary antibody (1/50,000).
Paraffin-embedded human breast cancer tissue stained for Casein Kinase 1 gamma 2 using ab230045 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for Casien Kinase 1 gamma 2 using ab230045 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230045 has not yet been referenced specifically in any publications.