Western blot abreview for Anti-Casein Kinase 1 gamma 2 antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount
90 µg
Specification
Human osteosarcoma U2OS cells
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: Immunopure Pierce anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: Around 50 kDa
Positive control
U2OS cells transfected with a plasmid expressing FLAG-tagged CK1G2 (+) or a plasmid expressing only FLAG (-)

Additional data

Additional Notes
The same band was picked with two other CK1 gamma2 antibodies tested (ab102554, ab70862). However, no band was detected for the endogenous CK1G2 (maybe not expressed in U2OS cells?)

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Submitted Apr 12 2013

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