Overview

  • Product name

    Anti-Casein Kinase 2 beta antibody [2F12F3]
    See all Casein Kinase 2 beta primary antibodies
  • Description

    Mouse monoclonal [2F12F3] to Casein Kinase 2 beta
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig
  • Immunogen

    Recombinant full length protein corresponding to Human Casein Kinase 2 beta aa 1-215.
    Sequence:

    MSSSEEVSWI SWFCGLRGNE FFCEVDEDYI QDKFNLTGLN EQVPHYRQAL DMILDLEPDE ELEDNPNQSD LIEQAAEMLY GLIHARYILT NRGIAQMLEK YQQGDFGYCP RVYCENQPML PIGLSDIPGE AMVKLYCPKC MDVYTPKSSR HHHTDGAYFG TGFPHMLFMV HPEYRPKRPA NQFVPRLYGF KIHPMAYQLQ LQAASNFKSP VKTIR


    Database link: P67870

  • Positive control

    • Human Casein Kinase 2 beta (AA: FULL(1-215)) recombinant protein, Casein Kinase 2 beta (AA: FULL(1-215))-hIgGFc transfected HEK293 cell lysate, Hela, Jurkat, K562, HepG2, C6, SK-N-SH, NTERA-2, MCF-7, NIH/3T3 cell lysate, MCF-7 cells, HeLa cells, Human cervical cancer tissues, Human colon cancer tissues

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2F12F3
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/200 - 1/1000.

Target

Images

  • All lanes : Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990) at 1/500 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : C6 cell lysate
    Lane 6 : SK-N-SH cell lysate
    Lane 7 : NTERA-2 cell lysate
    Lane 8 : MCF-7 cell lysate
    Lane 9 : NIH/3T3 cell lysate

    Predicted band size: 25 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Casein Kinase 2 beta with ab201990 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Casein Kinase 2 beta with ab201990 at 1/200 dilution.

  • Flow cytometric analysis of HeLa cells using ab201990 at 1/200 dilution (green) and negative control (red).

  • Immunofluorescence analysis of MCF-7 cells using ab201990 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • All lanes : Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Casein Kinase 2 beta (AA: FULL(1-215))-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 25 kDa

  • Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990) at 1/500 dilution + Human Casein Kinase 2 beta (AA: FULL(1-215)) recombinant protein

    Predicted band size: 25 kDa

References

This product has been referenced in:

  • Chen M  et al. Sex-specific activation of SK current by isoproterenol facilitates action potential triangulation and arrhythmogenesis in rabbit ventricles. J Physiol 596:4299-4322 (2018). Read more (PubMed: 29917243) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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