Overview

  • Product name

    Anti-Casein Kinase 2 beta antibody
    See all Casein Kinase 2 beta primary antibodies
  • Description

    Rabbit polyclonal to Casein Kinase 2 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Pig, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-215 of Human Casein Kinase 2 beta.

  • Positive control

    • WB: MCF7 and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells. IHC-P: Mouse ovary tissue; CL1-0 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab151784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Paraffin-embedded mouse ovary tissue stained for Casein Kinase 2 using ab151784 at 1/500 dilution in immunohistochemical analysis.

  • Anti-Casein Kinase 2 beta antibody (ab151784) at 1/500 dilution + MCF7 whole cell
    lysate at 30 µg

    Predicted band size: 24 kDa



    12% SDS PAGE
  • Anti-Casein Kinase 2 beta antibody (ab151784) at 1/5000 dilution + NIH3T3 whole cell
    lysate at 30 µg

    Predicted band size: 24 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Casein Kinase 2 beta with ab151784 at 1/500. Co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded CL1-0 xenograft, labeling Casein Kinase 2 beta with ab151784 antibody at 1/100.

References

ab151784 has not yet been referenced specifically in any publications.

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