Overview

  • Product name

    Anti-Casein Kinase 2 beta antibody
    See all Casein Kinase 2 beta primary antibodies
  • Description

    Goat polyclonal to Casein Kinase 2 beta
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Rabbit, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Fall armyworm
  • Immunogen

    Synthetic peptide corresponding to Human Casein Kinase 2 beta aa 37-48 (internal sequence) (Cysteine residue). (NP_001311.3).
    Sequence:

    C-TGLNEQVPHYRQ


    Database link: P67870

  • Positive control

    • WB: NIH/3T3, HeLa, K562, MCF7 and HepG2 cell lysates; Mouse, rat and pig testis lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223482 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).

Target

Images

  • All lanes : Anti-Casein Kinase 2 beta antibody (ab223482) at 1 µg/ml

    Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate (in RIPA buffer)
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (in RIPA buffer)
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate (in RIPA buffer)
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate (in RIPA buffer)
    Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 24 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • All lanes : Anti-Casein Kinase 2 beta antibody (ab223482) at 0.3 µg/ml

    Lane 1 : Mouse testis lysate (in RIPA buffer)
    Lane 2 : Rat testis lysate (in RIPA buffer)
    Lane 3 : Pig testis lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 24 kDa



    Primary incubation was 1 hour.

    Approx 25kDa band observed in Mouse testis, 26kDa in Rat testis and 28kDa in Pig testis lysates (calculated MW of 24.9kDa according to Mouse NP_001290374.1, Rat NP_001030315.1 and pig NP_001138849.1).

References

ab223482 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223482.
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