Anti-Casein Kinase 2 beta antibody (ab227500)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Casein Kinase 2 beta antibody
See all Casein Kinase 2 beta primary antibodies -
Description
Rabbit polyclonal to Casein Kinase 2 beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey -
Immunogen
Recombinant fragment within Human Casein Kinase 2 beta (internal sequence). The exact sequence is proprietary.
Database link: P67870 -
Positive control
- ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue. WB: U-87 MG whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/500 - 1/3000. Predicted molecular weight: 25 kDa. | |
IHC-P | 1/100 - 1/1000. | |
ICC/IF | 1/100 - 1/1000. |
Target
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Function
Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit. -
Sequence similarities
Belongs to the casein kinase 2 subunit beta family. -
Post-translational
modificationsPhosphorylated by alpha subunit. - Information by UniProt
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Database links
- Entrez Gene: 100873151 Chicken
- Entrez Gene: 539235 Cow
- Entrez Gene: 1460 Human
- Entrez Gene: 13001 Mouse
- Entrez Gene: 397211 Pig
- Entrez Gene: 100301531 Rabbit
- Entrez Gene: 81650 Rat
- Entrez Gene: 715807 Rhesus monkey
see all -
Alternative names
- Casein kinase 2 beta polypeptide antibody
- Casein kinase II beta subunit antibody
- Casein kinase II subunit beta antibody
see all
Images
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Anti-Casein Kinase 2 beta antibody (ab227500) at 1/500 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Predicted band size: 25 kDa12% SDS-PAGE gel.
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Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Casein Kinase 2 beta (green) using ab227500 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody (ab227500)
Paraffin-embedded human breast cancer tissue stained for Casein Kinase 2 beta using ab227500 at 1/250 dilution in immunohistochemical analysis.
References
ab227500 has not yet been referenced specifically in any publications.