Anti-Casein Kinase 2 beta antibody (ab227500)

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Key features and details

  • Rabbit polyclonal to Casein Kinase 2 beta
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Casein Kinase 2 beta antibody
    See all Casein Kinase 2 beta primary antibodies

  • Description

    Rabbit polyclonal to Casein Kinase 2 beta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey

  • Immunogen

    Recombinant fragment within Human Casein Kinase 2 beta (internal sequence). The exact sequence is proprietary.
    Database link: P67870

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue. WB: U-87 MG whole cell lysate.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Western blot - Anti-Casein Kinase 2 beta antibody (ab227500)
    Western blot - Anti-Casein Kinase 2 beta antibody (ab227500)
    Anti-Casein Kinase 2 beta antibody (ab227500) at 1/500 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

    Predicted band size: 25 kDa



    12% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody (ab227500)
    Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody (ab227500)

    Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Casein Kinase 2 beta (green) using ab227500 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody (ab227500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody (ab227500)

    Paraffin-embedded human breast cancer tissue stained for Casein Kinase 2 beta using ab227500 at 1/250 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab227500? Please let us know so that we can cite the reference in this datasheet.

ab227500 has not yet been referenced specifically in any publications.

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