Key features and details
- Rabbit polyclonal to Casein Kinase 2 beta
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Casein Kinase 2 beta antibody
See all Casein Kinase 2 beta primary antibodies
DescriptionRabbit polyclonal to Casein Kinase 2 beta
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
Recombinant fragment within Human Casein Kinase 2 beta (internal sequence). The exact sequence is proprietary.
Database link: P67870
- ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue. WB: U-87 MG whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionParticipates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
Sequence similaritiesBelongs to the casein kinase 2 subunit beta family.
modificationsPhosphorylated by alpha subunit.
- Information by UniProt
- Casein kinase 2 beta polypeptide antibody
- Casein kinase II beta subunit antibody
- Casein kinase II subunit beta antibody
Anti-Casein Kinase 2 beta antibody (ab227500) at 1/500 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Predicted band size: 25 kDa
12% SDS-PAGE gel.
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Casein Kinase 2 beta (green) using ab227500 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).
Paraffin-embedded human breast cancer tissue stained for Casein Kinase 2 beta using ab227500 at 1/250 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227500 has not yet been referenced specifically in any publications.