Anti-Casein Kinase 2 beta antibody (ab227500)

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Overview

  • Product name

    Anti-Casein Kinase 2 beta antibody
    See all Casein Kinase 2 beta primary antibodies

  • Description

    Rabbit polyclonal to Casein Kinase 2 beta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey

  • Immunogen

    Recombinant fragment within Human Casein Kinase 2 beta (internal sequence). The exact sequence is proprietary.
    Database link: P67870

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue. WB: U-87 MG whole cell lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Western blot - Anti-Casein Kinase 2 beta antibody (ab227500)
    Western blot - Anti-Casein Kinase 2 beta antibody (ab227500)
    Anti-Casein Kinase 2 beta antibody (ab227500) at 1/500 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

    Predicted band size: 25 kDa



    12% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody (ab227500)
    Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody (ab227500)

    Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Casein Kinase 2 beta (green) using ab227500 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody (ab227500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody (ab227500)

    Paraffin-embedded human breast cancer tissue stained for Casein Kinase 2 beta using ab227500 at 1/250 dilution in immunohistochemical analysis.

References

ab227500 has not yet been referenced specifically in any publications.

Publishing research using ab227500? Please let us know so that we can cite the reference in this datasheet.

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