Recombinant
RabMAb

Recombinant Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

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Overview

  • Product name

    Anti-Casein Kinase 2 beta antibody [EP1995Y]
    See all Casein Kinase 2 beta primary antibodies

  • Description

    Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Horse, Human

  • Immunogen

    Synthetic peptide within Human Casein Kinase 2 beta aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: K562 cell lysate ICC/IF: HeLa cells IHC-P: Human ovary carcinoma tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP1995Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 100 - 250 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
      Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
      Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
      Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
      Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

      Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
      Flow Cytometry - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
      Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
      Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)This image is courtesy of an anonymous Abreview
      Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + MEF whole cell lysate at 20 µg

      Secondary
      Alexa Fluor® 680-conjugated polyclonal goat anti-rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 25 kDa
      Observed band size: 25 kDa


      Exposure time: 10 minutes


      Blocked for 30 minutes at 25°C.

      Incubated with the primary antibody for 2 hours at 25°C in blocking buffer.

      See Abreview

    References

    Publishing research using ab76025? Please let us know so that we can cite the reference in this datasheet.

    ab76025 has been referenced in 5 publications.

    • Yang CP  et al. Comprehensive integrative analyses identify GLT8D1 and CSNK2B as schizophrenia risk genes. Nat Commun 9:838 (2018). PubMed: 29483533
    • Takai T  et al. Casein kinase 2 phosphorylates and stabilizes C/EBPß in pancreatic ß cells. Biochem Biophys Res Commun 497:451-456 (2018). IP . PubMed: 29448105
    • Borgo C  et al. Dependence of HSP27 cellular level on protein kinase CK2 discloses novel therapeutic strategies. Biochim Biophys Acta Gen Subj 1862:2902-2910 (2018). PubMed: 30279146
    • Borgo C  et al. CK2 modulates adipocyte insulin-signaling and is up-regulated in human obesity. Sci Rep 7:17569 (2017). PubMed: 29242563
    • Ferrer-Font L  et al. Targeting Protein Kinase CK2: Evaluating CX-4945 Potential for GL261 Glioblastoma Therapy in Immunocompetent Mice. Pharmaceuticals (Basel) 10:N/A (2017). PubMed: 28208677

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (ovary)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    ovary
    Blocking step
    BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Dr. qiuxia liang

    Verified customer

    Submitted Apr 06 2018

    Immunoprecipitation abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    U2OS cells
    Read More

    Abcam user community

    Verified customer

    Submitted May 12 2017

    Western blot abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    20 µg
    Specification
    MEF cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 03 2017

    Western blot abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    U2OS cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 16 2016

    Western blot abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
    Loading amount
    40 µg
    Specification
    C57BL/6 Mouse splenocytes
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Tomar Ghansah

    Verified customer

    Submitted Oct 05 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Horse Tissue sections (Mammary gland tissue)
    Specification
    Mammary gland tissue
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Read More

    Dr. Jan Spaas

    Verified customer

    Submitted Jun 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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