Recombinant Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
![Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.com/ps/products/76/ab76025/Images/ab76025-91330-anti-casein-kinase-2-beta-antibody-ep1995y-western-blot.gif "Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Horse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Casein Kinase 2 beta antibody [EP1995Y]
See all Casein Kinase 2 beta primary antibodies -
Description
Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Horse, Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Casein Kinase 2 beta aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: K562 cell lysate ICC/IF: HeLa cells IHC-P: Human ovary carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1995Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/500 - 1/2000. Predicted molecular weight: 25 kDa.
|
| IHC-P | (1) |
Use a concentration of 100 - 250 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| ICC/IF |
1/100 - 1/250.
|
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| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 25 kDa. |
|
IHC-P
Use a concentration of 100 - 250 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit. -
Sequence similarities
Belongs to the casein kinase 2 subunit beta family. -
Post-translational
modificationsPhosphorylated by alpha subunit. - Information by UniProt
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Database links
- Entrez Gene: 1460 Human
- Entrez Gene: 13001 Mouse
- Entrez Gene: 81650 Rat
- Omim: 115441 Human
- SwissProt: P67870 Human
- SwissProt: P67871 Mouse
- SwissProt: P67874 Rat
- Unigene: 73527 Human
see all -
Alternative names
- Casein kinase 2 beta polypeptide antibody
- Casein kinase II beta subunit antibody
- Casein kinase II subunit beta antibody
see all
Images
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Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.com/ps/products/76/ab76025/Images/ab76025-91329-anti-casein-kinase-2-beta-antibody-ep1995y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.com/ps/products/76/ab76025/Images/ab76025-91328-anti-casein-kinase-2-beta-antibody-ep1995y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.com/ps/products/76/ab76025/Images/ab76025-91327-anti-casein-kinase-2-beta-antibody-ep1995y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.com/ps/products/76/ab76025/Images/ab76025-280855-anti-casein-kinase-2-beta-antibody-ep1995y-western-blot.jpg)
Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)This image is courtesy of an anonymous AbreviewAnti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + MEF whole cell lysate at 20 µg
Secondary
Alexa Fluor® 680-conjugated polyclonal goat anti-rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 10 minutesBlocked for 30 minutes at 25°C.
Incubated with the primary antibody for 2 hours at 25°C in blocking buffer.
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![Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)](https://www.abcam.com/ps/products/76/ab76025/Images/ab76025-6-benefits-of-recombinant-antibodies.png)
Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab76025 has been referenced in 15 publications.
- Zonta F et al. Contribution of the CK2 Catalytic Isoforms a and a' to the Glycolytic Phenotype of Tumor Cells. Cells 10:N/A (2021). PubMed: 33477590
- D'Amore C et al. "Janus" efficacy of CX-5011: CK2 inhibition and methuosis induction by independent mechanisms. Biochim Biophys Acta Mol Cell Res 1867:118807 (2020). PubMed: 32745724
- Borgo C et al. A N-terminally deleted form of the CK2a' catalytic subunit is sufficient to support cell viability. Biochem Biophys Res Commun 531:409-415 (2020). PubMed: 32800562
- Sanna M et al. White Adipose Tissue Expansion in Multiple Symmetric Lipomatosis Is Associated with Upregulation of CK2, AKT and ERK1/2. Int J Mol Sci 21:N/A (2020). PubMed: 33114687
- Kim JM et al. A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation. Nat Commun 11:2289 (2020). PubMed: 32385263