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  1. Link

    casein-kinase-2-beta-antibody-ep1995y-ab76025.pdf

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RecombinantRabMAb

Recombinant Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (15)

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Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
  • Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
  • Flow Cytometry (Intracellular) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
  • Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
  • Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Horse, Human

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Overview

  • Product name

    Anti-Casein Kinase 2 beta antibody [EP1995Y]
    See all Casein Kinase 2 beta primary antibodies
  • Description

    Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Horse, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Casein Kinase 2 beta aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: K562 cell lysate ICC/IF: HeLa cells IHC-P: Human ovary carcinoma tissue
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1995Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Casein Kinase 2 beta antibody [EP1995Y] - BSA and Azide free (ab239872)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/500 - 1/2000. Predicted molecular weight: 25 kDa.
IHC-P (1)
Use a concentration of 100 - 250 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/500 - 1/2000. Predicted molecular weight: 25 kDa.
IHC-P
Use a concentration of 100 - 250 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes
Is unsuitable for IP.

Target

  • Function

    Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
  • Sequence similarities

    Belongs to the casein kinase 2 subunit beta family.
  • Post-translational
    modifications

    Phosphorylated by alpha subunit.
  • Target information above from: UniProt accession P67870 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1460 Human
    • Entrez Gene: 13001 Mouse
    • Entrez Gene: 81650 Rat
    • Omim: 115441 Human
    • SwissProt: P67870 Human
    • SwissProt: P67871 Mouse
    • SwissProt: P67874 Rat
    • Unigene: 73527 Human
    • Unigene: 378901 Mouse
    • Unigene: 137692 Rat
    see all
  • Alternative names

    • Casein kinase 2 beta polypeptide antibody
    • Casein kinase II beta subunit antibody
    • Casein kinase II subunit beta antibody
    • CK II beta antibody
    • CK2B antibody
    • CK2N antibody
    • CSK2B antibody
    • CSK2B_HUMAN antibody
    • CSNK 2B antibody
    • csnk2b antibody
    • G5A antibody
    • MGC138222 antibody
    • MGC138224 antibody
    • Phosvitin antibody
    • Protein G5a antibody
    see all

Images

  • Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

    Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Flow Cytometry (Intracellular) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

    Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

     

  • Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)This image is courtesy of an anonymous Abreview
    Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + MEF whole cell lysate at 20 µg

    Secondary
    Alexa Fluor® 680-conjugated polyclonal goat anti-rabbit IgG at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 10 minutes


    Blocked for 30 minutes at 25°C.

    Incubated with the primary antibody for 2 hours at 25°C in blocking buffer.

    See Abreview

  • Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (15)

Publishing research using ab76025? Please let us know so that we can cite the reference in this datasheet.

ab76025 has been referenced in 15 publications.

  • Zonta F  et al. Contribution of the CK2 Catalytic Isoforms a and a' to the Glycolytic Phenotype of Tumor Cells. Cells 10:N/A (2021). PubMed: 33477590
  • D'Amore C  et al. "Janus" efficacy of CX-5011: CK2 inhibition and methuosis induction by independent mechanisms. Biochim Biophys Acta Mol Cell Res 1867:118807 (2020). PubMed: 32745724
  • Borgo C  et al. A N-terminally deleted form of the CK2a' catalytic subunit is sufficient to support cell viability. Biochem Biophys Res Commun 531:409-415 (2020). PubMed: 32800562
  • Sanna M  et al. White Adipose Tissue Expansion in Multiple Symmetric Lipomatosis Is Associated with Upregulation of CK2, AKT and ERK1/2. Int J Mol Sci 21:N/A (2020). PubMed: 33114687
  • Kim JM  et al. A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation. Nat Commun 11:2289 (2020). PubMed: 32385263
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (ovary)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
30 µg
Specification
ovary
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Dr. qiuxia liang

Verified customer

Submitted Apr 06 2018

Immunoprecipitation abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
U2OS cells
Read More

Abcam user community

Verified customer

Submitted May 12 2017

Western blot abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
20 µg
Specification
MEF cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted May 03 2017

Western blot abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
U2OS cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Nov 16 2016

Western blot abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
Loading amount
40 µg
Specification
C57BL/6 Mouse splenocytes
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Tomar Ghansah

Verified customer

Submitted Oct 05 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Casein Kinase 2 beta antibody [EP1995Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Mammary gland tissue)
Specification
Mammary gland tissue
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Read More

Dr. Jan Spaas

Verified customer

Submitted Jun 09 2011

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