Recombinant Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Horse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Casein Kinase 2 beta antibody [EP1995Y]
See all Casein Kinase 2 beta primary antibodies -
Description
Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Horse, Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Casein Kinase 2 beta aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: K562 cell lysate ICC/IF: HeLa cells IHC-P: Human ovary carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1995Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/500 - 1/2000. Predicted molecular weight: 25 kDa.
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IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 25 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit. -
Sequence similarities
Belongs to the casein kinase 2 subunit beta family. -
Post-translational
modificationsPhosphorylated by alpha subunit. - Information by UniProt
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Database links
- Entrez Gene: 1460 Human
- Entrez Gene: 13001 Mouse
- Entrez Gene: 81650 Rat
- Omim: 115441 Human
- SwissProt: P67870 Human
- SwissProt: P67871 Mouse
- SwissProt: P67874 Rat
- Unigene: 73527 Human
see all -
Alternative names
- Casein kinase 2 beta polypeptide antibody
- Casein kinase II beta subunit antibody
- Casein kinase II subunit beta antibody
see all
Images
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Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.
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Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab76025 has been referenced in 18 publications.
- Park K et al. ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters. Nucleic Acids Res 50:3835-3851 (2022). PubMed: 35325203
- Asif M et al. De novo variants of CSNK2B cause a new intellectual disability-craniodigital syndrome by disrupting the canonical Wnt signaling pathway. HGG Adv 3:100111 (2022). PubMed: 35571680
- Zonta F et al. Contribution of the CK2 Catalytic Isoforms a and a' to the Glycolytic Phenotype of Tumor Cells. Cells 10:N/A (2021). PubMed: 33477590
- Lee JH et al. Poly-ADP-ribosylation drives loss of protein homeostasis in ATM and Mre11 deficiency. Mol Cell 81:1515-1533.e5 (2021). PubMed: 33571423
- D'Amore C et al. "Janus" efficacy of CX-5011: CK2 inhibition and methuosis induction by independent mechanisms. Biochim Biophys Acta Mol Cell Res 1867:118807 (2020). PubMed: 32745724