Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1994] to Casein Kinase 2 beta
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Casein Kinase 2 beta antibody [EPR1994]
See all Casein Kinase 2 beta primary antibodies
DescriptionRabbit monoclonal [EPR1994] to Casein Kinase 2 beta
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a region within Human Casein Kinase 2 beta (UniProt P67870).
- Human breast tissue; HepG2, HeLa, SH SY5Y and HUVEC cell lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.94 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use a HRP/AP polymerized secondary antibody.|
|WB||1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).|
FunctionParticipates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
Sequence similaritiesBelongs to the casein kinase 2 subunit beta family.
modificationsPhosphorylated by alpha subunit.
- Information by UniProt
- Casein kinase 2 beta polypeptide antibody
- Casein kinase II beta subunit antibody
- Casein kinase II subunit beta antibody
All lanes : Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH SY5Y cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of Casein Kinase 2 beta in formalin fixed, paraffin embedded Human breast tissue, using ab133576 at a 1/50 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133576 has been referenced in 3 publications.
- Kim JM et al. A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation. Nat Commun 11:2289 (2020). PubMed: 32385263
- Beale AD et al. Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells. J Biol Rhythms 34:144-153 (2019). PubMed: 30898060
- Miao Z et al. Comparative proteomic profile of the human placenta in normal and fetal growth restriction subjects. Cell Physiol Biochem 34:1701-10 (2014). PubMed: 25401993