Anti-Casein Kinase 2 beta (phospho S209) antibody (ab12861)

Overview

  • Product name

    Anti-Casein Kinase 2 beta (phospho S209) antibody
    See all Casein Kinase 2 beta primary antibodies
  • Description

    Rabbit polyclonal to Casein Kinase 2 beta (phospho S209)
  • Host species

    Rabbit
  • Specificity

    Does not cross react with Casein Kinase 2 alpha [pS360] (mouse), [pS362], [pS370], or Casein Kinase 2 beta pS2].
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic phosphopeptide (Human) from the region of casein kinase 2 that contains serine 209.

  • Positive control

    • HeLa cell lysates or K562 cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Casein Kinase 2 beta. The final product is generated by affinity chromatography using a Casein Kinase 2 beta derived peptide that is phosphorylated at serine 209.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 in 3% milk in 0.1% Tween20 (TBST). Incubate overnight. Detects a band of approximately 25 kDa. Block 1hr with 3% milk in 0.1% Tween20 (TBST).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
    • Sequence similarities

      Belongs to the casein kinase 2 subunit beta family.
    • Post-translational
      modifications

      Phosphorylated by alpha subunit.
    • Information by UniProt
    • Database links

    • Alternative names

      • Casein kinase 2 beta polypeptide antibody
      • Casein kinase II beta subunit antibody
      • Casein kinase II subunit beta antibody
      • CK II beta antibody
      • CK2B antibody
      • CK2N antibody
      • CSK2B antibody
      • CSK2B_HUMAN antibody
      • CSNK 2B antibody
      • csnk2b antibody
      • G5A antibody
      • MGC138222 antibody
      • MGC138224 antibody
      • Phosvitin antibody
      • Protein G5a antibody
      see all

    Images

    • Peptide Competition and Phosphatase Treatment

      Lysates prepared from HeLa cells were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with ab12861 antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method.

      The data show that only the peptide corresponding to ab12861 blocks the signal, verifying the specificity of the antibody.

      Peptide Competition and Phosphatase Treatment

    References

    ab12861 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for your enquiry. The S209 refers to serine 209. The immunogen used to generate this antibody was a synthetic phosphopeptide (Human) from the region of casein kinase 2 that contains serine 209. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Casein Kinase 2 beta. The final product is generated by affinity chromatography using a Casein Kinase 2 beta derived peptide that is phosphorylated at serine 209. Ab12861 has been tested for application in Western blotting but has not yet been tested in IF. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please contact us again.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up