Anti-CASK antibody (ab244393)
Key features and details
- Rabbit polyclonal to CASK
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CASK antibody
See all CASK primary antibodies -
Description
Rabbit polyclonal to CASK -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human CASK aa 250-450.
Database link: O70589-3 -
Positive control
- ICC/IF: U-251 MG cells. WB: RT4 cell lysate. IHC-P: Human duodenum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. -
Tissue specificity
Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver. -
Involvement in disease
Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) [MIM:300749]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Patients with mental retardation X-linked CASK-related can manifest a severe phenotype consisting of severe intellectual deficit, congenital or postnatal microcephaly, disproportionate brainstem and cerebellar hypoplasia (MICPCH Syndrome). A milder phenotype consists of mental retardation alone or associated with nystagmus.
Defects in CASK are the cause of FG syndrome type 4 (FGS4) [MIM:300422]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation. -
Sequence similarities
In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
Domain
The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.
The protein kinase domain mediates the interaction with FCHSD2. -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8573 Human
- Entrez Gene: 12361 Mouse
- Entrez Gene: 29647 Rat
- Omim: 300172 Human
- SwissProt: O14936 Human
- SwissProt: O70589 Mouse
- SwissProt: Q62915 Rat
- Unigene: 495984 Human
see all -
Alternative names
- CAGH39 antibody
- Caki antibody
- Calcium/calmodulin dependent serine protein kinase antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for CASK (green) using ab244393 at 4 µg/ml in ICC/IF.
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Paraffin-embedded human duodenum tissue stained for CASK using ab244393 at 1/20 dilution in immunohistochemical analysis.
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Paraffin-embedded human testis tissue stained for CASK using ab244393 at 1/20 dilution in immunohistochemical analysis.
Low expression as expected.
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Anti-CASK antibody (ab244393) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) cell lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab244393 has been referenced in 1 publication.
- Da Z et al. Bioinformatics combined with quantitative proteomics analyses and identification of potential biomarkers in cholangiocarcinoma. Cancer Cell Int 20:130 (2020). PubMed: 32336950