• Product name
  • Description
    Goat polyclonal to CASP
  • Host species
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CASP aa 2-14 (N terminal).


    (Peptide available as ab22893)

  • Positive control
    • Jurkat lysate.
  • General notes
    GenBank Accession Number – NP_004279.

    Protein previously labeled as PSCDBP.



Our Abpromise guarantee covers the use of ab2247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).Can be blocked with Human CASP peptide (ab22893).
ELISA 1/16000.


  • Function
    By its binding to cytohesin-1 (CYTH1), it modifies activation of ARFs by CYTH1 and its precise function may be to sequester CYTH1 in the cytoplasm.
  • Tissue specificity
    Expressed in lymph nodes, thymus, spleen, lung, peripheral blood leukocytes and bone marrow.
  • Sequence similarities
    Contains 1 PDZ (DHR) domain.
  • Cellular localization
    Cytoplasm. Early endosome. Recruited from the cytosol to endosomes by SNX27.
  • Information by UniProt
  • Database links
  • Alternative names
    • B3-1 antibody
    • B31 antibody
    • CASP antibody
    • Cbp HE antibody
    • CYBR antibody
    • CYTHIP antibody
    • Cytip antibody
    • CYTIP_HUMAN antibody
    • Cytohesin 1 interacting protein antibody
    • Cytohesin binder and regulator antibody
    • Cytohesin-associated scaffolding protein antibody
    • Cytohesin-binding protein HE antibody
    • Cytohesin-interacting protein antibody
    • HE antibody
    • Pleckstrin Homology Sec7 and Coiled-Coil Domains Binding Protein antibody
    • Pleckstrin homology Sec7 and coiled-coil domains-binding protein antibody
    • Pleckstrin homology, Sec7 and coiled/coil domains, binding protein antibody
    see all


  • ab2247 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2247 staining (1 µg/ml) of MOLT4 cell lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence


This product has been referenced in:
  • O'Brien M  et al. Upregulation of PSCDBP, TLR2, TWIST1, FLJ35382, EDNRB, and RGS12 gene expression in human myometrium at labor. Reprod Sci 15:382-93 (2008). Read more (PubMed: 18497345) »
  • MacNeil AJ  et al. Sorting nexin 27 interacts with the Cytohesin associated scaffolding protein (CASP) in lymphocytes. Biochem Biophys Res Commun 359:848-53 (2007). IP ; Human . Read more (PubMed: 17577583) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell (AGS human gastric carcinoma cells)
AGS human gastric carcinoma cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sandwich (Detection)

Abcam user community

Verified customer

Submitted Jul 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
AGS Gastric Carcinoma cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 17 2010


Thank you very much for your enquiry and patience. Below is the Western blotting protocol that the originator used with ab2247. I hope this helps and please let me know if you need further assistance. - Lysis. Cell pellets were washed with ice-cold PBS. 1 ml of RIPA buffer was added per 1E8 cells and incubated on ice for 20 min, vortexing 2-3 times, briefly. The lysate was aliquotted into 1.5 ml microfuge tubes and centrifuged at 13,000 rpm for 5 min in a microfuge. The supernatant was transferred into clean tubes and its protein concentration was measured with BioRad protein assay. The concentration was then adjusted to 5 mg/ml with RIPA lysis buffer. An equal volume of 2 x SDS sample buffer was then added and the cell lysate was boiled for 5 minutes. Lysates were stored at -80C until use.(RIPA buffer = 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 µg/ml Aprotinin, 5 µg/ml Leupeptin, 1% Triton X-100, 1% Sodium deoxycholate, 0.1% SDS). - SDS PAGE. Samples were run at 200V constant on a 12% acrylamide SDS-PAGE mini gel - using Biorad Mini-Protean 3 kit and protocols. Before loading samples had 5% (v/v) 2-ME added and were boiled for 3 minutes. - Transfer. We used a Biorad Mini Trans-Blot, constant 100 V for 1 hour. Transfer Buffer was 20 mM Tris pH 8.0, 150 mM Glycine, 10% Methanol. We transferred to Millipore PVDF membrane and stained with Ponceau Red to evaluate the transfer. - Staining. The membrane was blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05% Tween-20) for 1 hr at room temperature with agitation. Primary antibody was incubated for 1 hr at room temperature with agitation. We used sigma secondary (Sigma anti-goat-HRP Product # A4174, use 1:3000) for 1 hr at room temperature with agitation. We washed with TBST three times after primary and secondary antibody, each wash lasting for 5-10 mins. ECL-plus (Amersham) was used rather than ECL, which is considerably more sensitive. Final detection was on autoradiography film.

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