Overview

  • Product name
  • Description
    Rabbit polyclonal to Caspase-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Caspase-1 (internal sequence). Immunogen is within the p20 subunit.
    Database link: P29466

  • Positive control
    • Placenta

Properties

Applications

Our Abpromise guarantee covers the use of ab1872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. PubMed: 21228274
ICC/IF 1/200.

Target

  • Function
    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • Tissue specificity
    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

Images

  • ab1872 staining Caspase-1 in mouse immortalised bone marrow-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 (0.1%) and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1:200 in PBS + 0.1% BSA/5% goat serum) for 1 hour at 20°C. An anti-rabbit Alexa Fluor®568 (1/500) ab175471) was used as the secondary antibody.

    See Abreview

  • ab1872 staining Caspase-1 in formalin-fixed, paraffin-embedded Human placenta tissue by Immunohistochemistry.

  • Immunofluorescence analysis of caspase-1 in H9c2 cardiomyocytes treated with control, medium and high glucose using ab1872 (1:50) ; scale bar: 20 µm. 
    Ctrl: control glucose, 5.6 mM glucose for 36 h;
    MG: medium glucose, 25 mM glucose for 36 h;
    HG: high glucose, 33.3 mM glucose for 36 h; 

     

References

This product has been referenced in:
  • Ding HG  et al. Hypercapnia induces IL-1ß overproduction via activation of NLRP3 inflammasome: implication in cognitive impairment in hypoxemic adult rats. J Neuroinflammation 15:4 (2018). Read more (PubMed: 29304864) »
  • Feng J  et al. Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes. J Neuroinflammation 15:23 (2018). Read more (PubMed: 29357878) »

See all 19 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 13 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 24 2018

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19 cells)
Gel Running Conditions
Reduced Denaturing (4-12% bolt bis-tris gel)
Loading amount
15 µg
Treatment
125 ug/ml Cigarette Smoke Extract, 12-24 hr
Specification
ARPE-19 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 13 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization
No
Specification
Colon
Blocking step
Serum as blocking agent for 8 minute(s) · Concentration: 25% · Temperature: 37°C
Fixative
Glutaraldehyde
Username

Ms. Maria Daskalaki

Verified customer

Submitted Nov 01 2015

Application
Western blot
Sample
Rhesus monkey Tissue lysate - whole (lympoid node)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
40 µg
Specification
lympoid node
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 03 2015

Thank you for this clarification. Our datasheet and tissue specificity is based off of the findings reported in the literature. However, should we find that brain expression becomes reported in the literature we would make an effort to update our records.

Thank you for your reply. I apologize for my error, this is a caspase 1 antibody. The prostate cancer sounds like a good control. We also have lsited placenta. One recommendation might be to switch your blocking agent and increase the time to 2 hours. ...

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Thank you for contacting us.
For IHC on Frozen sections we recommend to use this product without any antigen reetrieval and develop using the ABC method. The primary antibody should be used at a dilution of 1/25 to 1/50 overnight at 4 degrees. For...

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Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching our que...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (immortalised bone marrow-derived macrophages)
Specification
immortalised bone marrow-derived macrophages
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. James Harris

Verified customer

Submitted Feb 01 2011

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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