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    caspase-1-antibody-ab62698.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Anti-Caspase-1 antibody (ab62698)

  • Datasheet
Reviews (1)Q&A (1)References (9)

Product price, shipping and contact information

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (ab62698)
  • Western blot - Anti-Caspase-1 antibody (ab62698)

Key features and details

  • Rabbit polyclonal to Caspase-1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Caspase-1 antibody
    See all Caspase-1 primary antibodies
  • Description

    Rabbit polyclonal to Caspase-1
  • Host species

    Rabbit
  • Specificity

    ab62698 recognizes Caspase-1 subunit p10.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-1.
    Database link: P29466

  • Positive control

    • Human brain tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab62698 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Caspase-1 protein (Active) (ab39901)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab62698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/1000.
IHC-P
1/50 - 1/100.
Notes
WB
1/500 - 1/1000.
IHC-P
1/50 - 1/100.

Target

  • Function

    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • Tissue specificity

    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications

    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P29466 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 834 Human
    • Omim: 147678 Human
    • SwissProt: P29466 Human
    • Unigene: 2490 Human
    • Alternative names

      • CASP-1 antibody
      • CASP1 antibody
      • CASP1_HUMAN antibody
      • Caspase 1 antibody
      • Caspase-1 subunit p10 antibody
      • ICE antibody
      • IL-1 beta-converting enzyme antibody
      • IL-1BC antibody
      • IL1 beta converting enzyme antibody
      • IL1B convertase antibody
      • Interleukin 1 beta convertase antibody
      • Interleukin 1B converting enzyme antibody
      • Interleukin-1 beta convertase antibody
      • Interleukin-1 beta-converting enzyme antibody
      • p45 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (ab62698)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (ab62698)

      ab62698 at a 1:50 dilution, staining Caspase-1 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.

    • Western blot - Anti-Caspase-1 antibody (ab62698)
      Western blot - Anti-Caspase-1 antibody (ab62698)
      All lanes : Anti-Caspase-1 antibody (ab62698)

      Lane 1 : 293 cell extract
      Lane 2 : MCF-7 cell extract
      Lane 3 : HeLa cell extract
      Lane 4 : 293 cell extract with blocking peptide

      Observed band size: 29 kDa
      why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (9)

    Publishing research using ab62698? Please let us know so that we can cite the reference in this datasheet.

    ab62698 has been referenced in 9 publications.

    • Chen H  et al. Sinomenine Attenuates Acetaminophen-Induced Acute Liver Injury by Decreasing Oxidative Stress and Inflammatory Response via Regulating TGF-ß/Smad Pathway in vitro and in vivo. Drug Des Devel Ther 14:2393-2403 (2020). PubMed: 32606606
    • Huang C  et al. TSPYL5 activates endoplasmic reticulum stress to inhibit cell proliferation, migration and invasion in colorectal cancer. Oncol Rep 44:449-456 (2020). PubMed: 32627024
    • Ding PH  et al. Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1ß Release Depend on Caspase-4. Front Microbiol 11:1881 (2020). PubMed: 32903638
    • Ying Y  et al. NLRP3 Inflammasome Activation by MicroRNA-495 Promoter Methylation May Contribute to the Progression of Acute Lung Injury. Mol Ther Nucleic Acids 18:801-814 (2019). PubMed: 31734560
    • Gao Q & Zhu H The Overexpression of Sirtuin1 (SIRT1) Alleviated Lipopolysaccharide (LPS)-Induced Acute Kidney Injury (AKI) via Inhibiting the Activation of Nucleotide-Binding Oligomerization Domain-Like Receptors (NLR) Family Pyrin Domain Containing 3 (NLRP3) Inflammasome. Med Sci Monit 25:2718-2726 (2019). PubMed: 30980521
    • Zheng B  et al. Administration of Dexmedetomidine inhibited NLRP3 inflammasome and microglial cell activities in hippocampus of traumatic brain injury rats. Biosci Rep 38:N/A (2018). PubMed: 30232232
    • Li YH  et al. In vivo effects of the NLRP1/NLRP3 inflammasome pathway on latent respiratory virus infection. Int J Mol Med 41:3620-3628 (2018). PubMed: 29512688
    • Li J  et al. LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation. Biosci Rep N/A:N/A (2017). PubMed: 29229673
    • Hadadi E  et al. Differential IL-1ß secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability. Sci Rep 6:39035 (2016). Flow Cyt . PubMed: 27976724

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Caspase-1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rhesus monkey Tissue sections (carotid artery)
    Permeabilization
    No
    Specification
    carotid artery
    Blocking step
    Protein block serum-free (Dako) as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 17 2019

    Question

    I am interested in your product anti-caspase 1 antibody (ab62698). Can you give me some additionnal information? Is there BSA present in the disolving buffer? Does the antibody recognizes the active form of human/mouse caspase 1? Is there literature available on the usage of this antibody in ELISA and/or IP?  

    Read More

    Abcam community

    Verified customer

    Asked on Nov 16 2011

    Answer

    Thank you for your inquiry. I can confirm that there is no BSA in the buffer and that the antibody recognizes Caspase-1 subunit p10.  There are no references citing the usage of this antibody in ELISA or IP.  As far as we're aware, this antibody has only been tested in WB, ELISA and IHC-P and not yet in IP.  Since this antibody is not yet tested in IP, I can offer a discount off a future purchase if you buy ab62698 now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab62698 in IP.  I will then send a discount code. This code must be issued before purchasing ab62698 so please wait for my reply before ordering. 2. Purchase ab62698 either by phone, fax, or online (www.abcam.com). 3. Test it in IP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab62698 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.    

    Read More

    Abcam Scientific Support

    Answered on Nov 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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