Anti-Caspase-1 antibody (ab62698)

Reviews (1)Q&A (1)References (5)

Overview

  • Product name

    Anti-Caspase-1 antibody
    See all Caspase-1 primary antibodies

  • Description

    Rabbit polyclonal to Caspase-1

  • Host species

    Rabbit

  • Specificity

    ab62698 recognizes Caspase-1 subunit p10.

  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide corresponding to Human Caspase-1.
    Database link: P29466

  • Positive control

    • Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • Function

    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

  • Tissue specificity

    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.

  • Post-translational
    modifications

    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • CASP-1 antibody
      • CASP1 antibody
      • CASP1_HUMAN antibody
      • Caspase 1 antibody
      • Caspase-1 subunit p10 antibody
      • ICE antibody
      • IL-1 beta-converting enzyme antibody
      • IL-1BC antibody
      • IL1 beta converting enzyme antibody
      • IL1B convertase antibody
      • Interleukin 1 beta convertase antibody
      • Interleukin 1B converting enzyme antibody
      • Interleukin-1 beta convertase antibody
      • Interleukin-1 beta-converting enzyme antibody
      • p45 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (ab62698)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (ab62698)

      ab62698 at a 1:50 dilution, staining Caspase-1 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.

    • Western blot - Anti-Caspase-1 antibody (ab62698)
      Western blot - Anti-Caspase-1 antibody (ab62698)
      All lanes : Anti-Caspase-1 antibody (ab62698)

      Lane 1 : 293 cell extract
      Lane 2 : MCF-7 cell extract
      Lane 3 : HeLa cell extract
      Lane 4 : 293 cell extract with blocking peptide

      Observed band size: 29 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Gao Q & Zhu H The Overexpression of Sirtuin1 (SIRT1) Alleviated Lipopolysaccharide (LPS)-Induced Acute Kidney Injury (AKI) via Inhibiting the Activation of Nucleotide-Binding Oligomerization Domain-Like Receptors (NLR) Family Pyrin Domain Containing 3 (NLRP3) Inflammasome. Med Sci Monit 25:2718-2726 (2019). Read more (PubMed: 30980521) »
    • Zheng B  et al. Administration of Dexmedetomidine inhibited NLRP3 inflammasome and microglial cell activities in hippocampus of traumatic brain injury rats. Biosci Rep 38:N/A (2018). Read more (PubMed: 30232232) »
    See all 5 Publications for this product

    Publishing research using ab62698? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Caspase-1 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rhesus monkey Tissue sections (carotid artery)
    Permeabilization
    No
    Specification
    carotid artery
    Blocking step
    Protein block serum-free (Dako) as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 17 2019

    Question

    I am interested in your product anti-caspase 1 antibody (ab62698). Can you give me some additionnal information? Is there BSA present in the disolving buffer? Does the antibody recognizes the active form of human/mouse caspase 1? Is there literature available on the usage of this antibody in ELISA and/or IP?  

    Read More
    Answer

    Thank you for your inquiry. I can confirm that there is no BSA in the buffer and that the antibody recognizes Caspase-1 subunit p10.  There are no references citing the usage of this antibody in ELISA or IP.  As far as we're aware, this antibody has only been tested in WB, ELISA and IHC-P and not yet in IP.  Since this antibody is not yet tested in IP, I can offer a discount off a future purchase if you buy ab62698 now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab62698 in IP.  I will then send a discount code. This code must be issued before purchasing ab62698 so please wait for my reply before ordering. 2. Purchase ab62698 either by phone, fax, or online (www.abcam.com). 3. Test it in IP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab62698 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.    

    Read More

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