Overview

  • Product name
    Anti-Caspase-1 antibody
    See all Caspase-1 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-1
  • Host species
    Rabbit
  • Specificity
    ab62698 recognizes Caspase-1 subunit p10.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-1.
    Database link: P29466

  • Positive control
    • Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • Tissue specificity
    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • CASP-1 antibody
      • CASP1 antibody
      • CASP1_HUMAN antibody
      • Caspase 1 antibody
      • Caspase-1 subunit p10 antibody
      • ICE antibody
      • IL-1 beta-converting enzyme antibody
      • IL-1BC antibody
      • IL1 beta converting enzyme antibody
      • IL1B convertase antibody
      • Interleukin 1 beta convertase antibody
      • Interleukin 1B converting enzyme antibody
      • Interleukin-1 beta convertase antibody
      • Interleukin-1 beta-converting enzyme antibody
      • p45 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (ab62698)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-1 antibody (ab62698)

      ab62698 at a 1:50 dilution, staining Caspase-1 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.

    • Western blot - Anti-Caspase-1 antibody (ab62698)
      Western blot - Anti-Caspase-1 antibody (ab62698)
      All lanes : Anti-Caspase-1 antibody (ab62698)

      Lane 1 : 293 cell extract
      Lane 2 : MCF-7 cell extract
      Lane 3 : HeLa cell extract
      Lane 4 : 293 cell extract with blocking peptide

      Observed band size: 29 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Hadadi E  et al. Differential IL-1ß secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability. Sci Rep 6:39035 (2016). Flow Cyt . Read more (PubMed: 27976724) »

    See 1 Publication for this product

    Publishing research using ab62698? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    I am interested in your product anti-caspase 1 antibody (ab62698). Can you give me some additionnal information? Is there BSA present in the disolving buffer? Does the antibody recognizes the active form of human/mouse caspase 1? Is there literature av...

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    Thank you for your inquiry. I can confirm that there is no BSA in the buffer and that the antibody recognizes Caspase-1 subunit p10.  There are no references citing the usage of this antibody in ELISA or IP.  As far as we're aware, this antibody ha...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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