Product nameAnti-Caspase-1 antibody
See all Caspase-1 primary antibodies
DescriptionRabbit polyclonal to Caspase-1
Specificityab62698 recognizes Caspase-1 subunit p10.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human Caspase-1.
Database link: P29466
- Human brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityProtein A purified
Purification notesab62698 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab62698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000.|
|IHC-P||1/50 - 1/100.|
FunctionThiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Tissue specificityExpressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism.
- Information by UniProt
- CASP-1 antibody
- CASP1 antibody
- CASP1_HUMAN antibody
ab62698 at a 1:50 dilution, staining Caspase-1 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
All lanes : Anti-Caspase-1 antibody (ab62698)
Lane 1 : 293 cell extract
Lane 2 : MCF-7 cell extract
Lane 3 : HeLa cell extract
Lane 4 : 293 cell extract with blocking peptide
Observed band size: 29 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Gao Q & Zhu H The Overexpression of Sirtuin1 (SIRT1) Alleviated Lipopolysaccharide (LPS)-Induced Acute Kidney Injury (AKI) via Inhibiting the Activation of Nucleotide-Binding Oligomerization Domain-Like Receptors (NLR) Family Pyrin Domain Containing 3 (NLRP3) Inflammasome. Med Sci Monit 25:2718-2726 (2019). Read more (PubMed: 30980521) »
- Zheng B et al. Administration of Dexmedetomidine inhibited NLRP3 inflammasome and microglial cell activities in hippocampus of traumatic brain injury rats. Biosci Rep 38:N/A (2018). Read more (PubMed: 30232232) »