Anti-Caspase-1 antibody (ab74279)
Key features and details
- Rabbit polyclonal to Caspase-1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caspase-1 antibody
See all Caspase-1 primary antibodies -
Description
Rabbit polyclonal to Caspase-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Caspase-1 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: P29466 -
Positive control
- Jurkat cells. Human placenta.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
Notes |
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
Target
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Function
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. -
Tissue specificity
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 834 Human
- Entrez Gene: 12362 Mouse
- Entrez Gene: 25166 Rat
- Omim: 147678 Human
- SwissProt: P29466 Human
- SwissProt: P29452 Mouse
- SwissProt: P43527 Rat
- Unigene: 2490 Human
see all -
Alternative names
- CASP-1 antibody
- CASP1 antibody
- CASP1_HUMAN antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab74279 has been referenced in 9 publications.
- Cui LJ et al. Pectic polysaccharides ameliorate the pathology of ulcerative colitis in mice by reducing pyroptosis. Ann Transl Med 10:347 (2022). PubMed: 35434032
- Zhang B et al. Exosomal miR-27b-3p Derived from Hypoxic Cardiac Microvascular Endothelial Cells Alleviates Rat Myocardial Ischemia/Reperfusion Injury through Inhibiting Oxidative Stress-Induced Pyroptosis via Foxo1/GSDMD Signaling. Oxid Med Cell Longev 2022:8215842 (2022). PubMed: 35847592
- Cao H et al. Inhibition of long non-coding RNA HOXA11-AS against neuroinflammation in Parkinson's disease model via targeting miR-124-3p mediated FSTL1/NF-?B axis. Aging (Albany NY) 13:11455-11469 (2021). PubMed: 33839699
- Liu F et al. Maxing Shigan Decoction Mitigates Mycoplasma pneumonia-Induced Pyroptosis in A549 Cells via the NLRP3 Inflammasome. Infect Drug Resist 14:859-867 (2021). PubMed: 33688221
- Zhao J et al. Lipoxin A4 promotes autophagy and inhibits overactivation of macrophage inflammasome activity induced by Pg LPS. J Int Med Res 49:300060520981259 (2021). PubMed: 33528285
- Jiang R et al. Ligustrazine Alleviate Acute Lung Injury Through Suppressing Pyroptosis and Apoptosis of Alveolar Macrophages. Front Pharmacol 12:680512 (2021). PubMed: 34122107
- Yan A et al. Activated protein C overexpression suppresses the pyroptosis of subarachnoid hemorrhage model cells by regulating the NLRP3 inflammasome pathway. Exp Ther Med 22:1391 (2021). PubMed: 34650639
- Gao Y et al. Induction of autophagy protects human dental pulp cells from lipopolysaccharide-induced pyroptotic cell death. Exp Ther Med 19:2202-2210 (2020). PubMed: 32104285
- Polcyn R et al. Enolase inhibition alters metabolic hormones and inflammatory factors to promote neuroprotection in spinal cord injury. Neurochem Int 139:104788 (2020). PubMed: 32650031