Overview

  • Product name

  • Description

    Rabbit polyclonal to Caspase-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Caspase-1 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P29466

  • Positive control

    • Jurkat cells. Human placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab74279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/200. Predicted molecular weight: 45 kDa.
IP 1/100 - 1/200.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function

    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • Tissue specificity

    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications

    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

Images

  • ab74279, at 1:50 dilution, staining Caspase-1 in formalin-fixed, paraffin-embedded human placenta tissue by immunohistochemistry.

References

ab74279 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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