Overview

  • Product name

    Caspase-1 Inhibitor Assay Kit
    See all Caspase-1 kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Assay time

    1h 30m
  • Product overview

    Abcam's Caspase 1 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate YVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-1 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-1 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • Platform

    Microplate reader

Properties

Images

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using YVAD-AFC as caspase 1 substrate and following Caspase 1 Inhibitor Drug Detection Kit (ab102489) protocol. Lanes:

    1.- Background Control: no Caspase 1, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 1, +z-VAD-FMK

    3.- Positive Control: + Caspase 1, no z-VAD-FMK

    4.- Caspase 1 + 2.5µM z-VAD-FMK

    5.- Caspase 1 + 10µM z-VAD-FMK

    6.- Caspase 1 + 40µM z-VAD-FMK

Protocols

References

This product has been referenced in:

  • Gren ST  et al. The protease inhibitor cystatin C down-regulates the release of IL-ß and TNF-a in lipopolysaccharide activated monocytes. J Leukoc Biol 100:811-822 (2016). Read more (PubMed: 27190304) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your telephone enquiry.

I can confirm this kit is designed to screen caspase-1 inhibitors. This can be done in 2 ways:

Putative caspase-1 inhibitors used to treat cells expressing caspase-1 can be compared for the inhibition potential. This assay does not need live cells but cell lysate can be used. In this case, cells can be parallel treated with the model inhibitor Z-VAD-FMK for comparison.

The kit comes with an active caspase-1 and compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-1 activity can be determined by comparison. Z-VAD-FMK can be used to compare other inhibitors of interest.


Citation: Wei Y. et al. (2006) Am. J. Physiol. Endocrinol. Metab. E 275-E281.


Hope this information is helpful. Please do not hesitate to contact us if you have any further questions or concerns.

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