Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
Product nameCaspase-1 Inhibitor Assay Kit
See all Caspase-1 kits
Sample typeAdherent cells, Suspension cells
Assay time1h 30m
Abcam's Caspase 1 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate YVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-1 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-1 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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This product is manufactured by BioVision, an Abcam company and was previously called K151 Caspase-1 Inhibitor Drug Screening Kit (Fluorometric). K151-100 is the same size as the 100 test size of ab102489.
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests 2X Reaction Buffer 1 x 10ml Active Caspase-1 1 x 100 units Caspase Inhibitor, Z-VAD-FMK 1 x 10µl Caspase Substrate YVAD-AFC 1 x 0.5ml DTT 1 x 100µl
RelevanceCaspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis.
- CASP1 nirs variant 1
- Z-LE(OMe)VD(OMe)-FMK, derivative of Z-LEVD-FMK (ab120489)
- Zaprinast, cGMP phosphodiesterase inhibitor (ab120940)
- Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)
- Z-WEHD-FMK, caspase-1, caspase-5 and cathepsin B inhibitor (ab142027)
- Z-AEVD-FMK, caspase-10 and related caspases inhibitor (ab142028)
Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using YVAD-AFC as caspase 1 substrate and following Caspase 1 Inhibitor Drug Detection Kit (ab102489) protocol. Lanes:
1.- Background Control: no Caspase 1, no z-VMAD-FMK
2.- Positive Inhibition Control: no Caspase 1, +z-VAD-FMK
3.- Positive Control: + Caspase 1, no z-VAD-FMK
4.- Caspase 1 + 2.5µM z-VAD-FMK
5.- Caspase 1 + 10µM z-VAD-FMK
6.- Caspase 1 + 40µM z-VAD-FMK
ab102489 has been referenced in 1 publication.
- Gren ST et al. The protease inhibitor cystatin C down-regulates the release of IL-ß and TNF-a in lipopolysaccharide activated monocytes. J Leukoc Biol 100:811-822 (2016). PubMed: 27190304