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Caspase 10 (active) Staining Kit - Green Fluorescence (ab219937) is a sensitive fluorometric assay to measure caspase 10 activation in live cells. The assay uses FAM-AEVD-FMK, which binds irreversibly to active caspase 10 in apoptotic cells. The fluorescent intensity of the FAM-AEVD-FMK signal is proportional to the amount of active caspase 10 and can be easily detected at Ex/Em = 490/525 nm by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.
Caspase activity assay kits are based on fluorescent inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. Caspase 10 plays an important role in death receptor signaling and apoptosis induction. It has been proven that caspase 10 has substrate selectivity for the peptide sequence Ala-Glu-Val-Asp (AEVD). This kit uses FAM-AEVD-FMK as a fluorescent indicator for caspase 10 activity. FAM-AEVD-FMK irreversibly binds to activated caspase 10 in apoptotic cells. Once bound to caspase 10, the fluorescent reagent is retained inside the cell. The binding event inhibits caspase 10 but will not stop apoptosis from proceeding.
|500X Hoechst||1 vial|
|500X Propidium Iodide||1 vial|
|Washing Buffer||1 x 100ml|
Our Abpromise guarantee covers the use of ab219937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
Detection of active Caspase 10 in Jurkat cells. Jurkat cells (3 x 105 cells/100 μL/well) were either untreated (control) or treated with 1 μM staurosporine for 3 hours. Cells were incubated with FAM-AEVD-FMK for 1 hour at 37°C. The fluorescent signal was measured at Ex/Em = 490/525 nm (cut off at 515 nm) with a FlexStation microplate reader (Molecular Devices) using bottom read mode.
Active caspase 10 staining in Jurkat cells. cells (3 x 105 cells/100 μL/well) were either untreated (A) or treated with 1 μM staurosporine for 3 hours (B). Cells were incubated with FAM-AEVD-FMK for 1 hour at 37°C. Increase in fluorescent intensity was observed using a fluorescence microscope with a FITC channel
ab219937 has not yet been referenced specifically in any publications.
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