Recombinant
RabMAb

Anti-Caspase-10 antibody [EPR10890] (ab177475)

Overview

  • Product name
    Anti-Caspase-10 antibody [EPR10890]
    See all Caspase-10 primary antibodies
  • Description
    Rabbit monoclonal [EPR10890] to Caspase-10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Caspase-10. The exact sequence is proprietary.
    Database link: Q92851

  • Positive control
    • K562 and Jurkat cell lysates; Human hepatocellular carcinoma tissue; K562 cells.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
      -AMC and Asp-Glu-Val-Asp-
      -AMC.
      Isoform C is proteolytically inactive.
    • Tissue specificity
      Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
    • Involvement in disease
      Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
      Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
      Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
    • Sequence similarities
      Belongs to the peptidase C14A family.
      Contains 2 DED (death effector) domains.
    • Post-translational
      modifications
      Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALPS2 antibody
      • Apoptotic protease Mch-4 antibody
      • CASP 10 antibody
      • CASP-10 antibody
      • CASP10 antibody
      • CASPA_HUMAN antibody
      • Caspase 10 apoptosis related cysteine peptidase antibody
      • Caspase-10 subunit p12 antibody
      • FADD like ICE2 antibody
      • Fas associated death domain protein antibody
      • FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody
      • FLICE 2 antibody
      • FLICE2 antibody
      • ICE like apoptotic protease 4 antibody
      • ICE-like apoptotic protease 4 antibody
      • Interleukin 1B converting enzyme 2 antibody
      • MCH 4 antibody
      see all

    Images

    • All lanes : Anti-Caspase-10 antibody [EPR10890] (ab177475) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 58 kDa

    • Western blot analysis on immunoprecipitation pellet from (1) K562 cell lysate or (2) 1XPBS (negative control) using ab177475 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caspase-10 with ab177475 at 1/50 dilution.

    • Flow cytometric analysis of permeabilized K562 cells labeling Caspase 10 with ab177475 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MOLT4)
    Gel Running Conditions
    Reduced Denaturing (4-20% gradient)
    Loading amount
    100000 cells
    Treatment
    Bortezomib 0-20hours
    Specification
    MOLT4
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 19 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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