• Product name

    Anti-Caspase-10/CASP-10 antibody
    See all Caspase-10/CASP-10 primary antibodies
  • Description

    Rabbit polyclonal to Caspase-10/CASP-10
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-10/CASP-10 aa 505-521 (C terminal).


    Database link: Q92851
    (Peptide available as ab8377)

  • Positive control

    • HeLa whole cell lysate.
  • General notes

    Previously labelled as Caspase-10. 



Our Abpromise guarantee covers the use of ab2012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ICC/IF 1/50. Fix with PFA.


  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
    -AMC and Asp-Glu-Val-Asp-
    Isoform C is proteolytically inactive.
  • Tissue specificity

    Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
  • Involvement in disease

    Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
    Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
    Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • Post-translational

    Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALPS2 antibody
    • Apoptotic protease Mch-4 antibody
    • CASP 10 antibody
    • CASP-10 antibody
    • CASP10 antibody
    • CASPA_HUMAN antibody
    • Caspase 10 apoptosis related cysteine peptidase antibody
    • Caspase-10 antibody
    • Caspase-10 subunit p12 antibody
    • FADD like ICE2 antibody
    • Fas associated death domain protein antibody
    • FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody
    • FLICE 2 antibody
    • FLICE2 antibody
    • ICE like apoptotic protease 4 antibody
    • ICE-like apoptotic protease 4 antibody
    • Interleukin 1B converting enzyme 2 antibody
    • MCH 4 antibody
    see all


  • Immunocytochemistry/Immunofluorescence of HeLa cells labeling Caspase-10/CASP-10 with Anti-Caspase-10/CASP-10 antibody (ab2012) at 5μg/ml.

  • All lanes : Anti-Caspase-10/CASP-10 antibody (ab2012) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : K562 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

  • ab2012 at 1:50 staining cultured mouse hippocampal astrocytes by ICC/IF. The astrocytes were maintained in culture for 2 days in the presence of 10% serum, fixed with 4% PFA in PBS for 15 min at room temperature and permeabilized for 5 min in 0.25% Triton X-100 in PBS. The tissue was incuabated with the antibody for 2 hours at room temperature. A Cy3 ® conjugated donkey anti-rabbit IgG (H+L) was used as the secondary.

    See Abreview


This product has been referenced in:

  • Pagnan G  et al. The combined therapeutic effects of bortezomib and fenretinide on neuroblastoma cells involve endoplasmic reticulum stress response. Clin Cancer Res 15:1199-209 (2009). WB ; Human . Read more (PubMed: 19228726) »
  • Green KA  et al. Caspase-mediated Cleavage of Insulin Receptor Substrate. J Biol Chem 279:25149-56 (2004). Read more (PubMed: 15069074) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Thank you for your interest in our products. Although it has been years since the last enquiry, this product has yet to be tested in immunohistochemistry. However, we have just bought a new facility in the UK, equipped with high tech machines, that will facilitate faster testing and imaging of products in IHC, WB, ELISA, FACS, and etc. Please rest assured that our goal is to have all products tested in at least IHC, WB, and ELISA but our facility is currently under staff, and trying to cope with all the requests that we had from customers as well as trying to maintain a constant release of new products. Please be patient with us until we can fully churn out more info for each product. If you need to use this product urgently, then may I encourage you make use of our Abreview system. We always encourage customers to send their results back to us, whether positive or negative. Each Abreview will be rewarded Abpoints, which can be used for discounts off future purchases and gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews Thank you for your feedback and for being our loyal customer.

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Immunocytochemistry/ Immunofluorescence
Mouse Cell (cultured hippocampal astrocytes)
cultured hippocampal astrocytes
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1%

Abcam user community

Verified customer

Submitted Mar 08 2007


We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

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As far as we are aware, Rabbit polyclonal to Caspase 10 (C-term) (ab2012), has not been tested in IHC.

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Yes, anti-caspase-10 is mouse reactive. 3T3 cell lysate was tested by Western blot. $195 /100 µg + $25 for FedEx overnight shipping.

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