Key features and details
- Rabbit polyclonal to Caspase-10/CASP-10
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Caspase-10/CASP-10 antibody
See all Caspase-10/CASP-10 primary antibodies
DescriptionRabbit polyclonal to Caspase-10/CASP-10
ab25045 recognises the proform, intermediates and cleaved form of caspase-10/CASP-10.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Jurkat cells induced by camptothecin (2uM/ml).
Previously labelled as Caspase-10.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab25045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 20, 30, 34 , 55 kDa.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
-AMC and Asp-Glu-Val-Asp-
Isoform C is proteolytically inactive.
Tissue specificityDetectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
Involvement in diseaseDefects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
modificationsCleavage by granzyme B and autocatalytic activity generate the two active subunits.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- ALPS2 antibody
- Apoptotic protease Mch-4 antibody
- CASP 10 antibody
Anti-Caspase-10/CASP-10 antibody (ab25045) + 2 microM/ml camptothecin treated Jurkat cells.
Predicted band size: 20, 30, 34 , 55 kDa
Observed band size: 20,30,34,55 kDa why is the actual band size different from the predicted?
ab25045 has been referenced in 1 publication.
- Seko Y et al. Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci Rep 5:13737 (2015). PubMed: 26348594