Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10890] to Caspase-10/CASP-10
- Suitable for: WB, IHC-P, IP, Flow Cyt
- Reacts with: Human
Product nameAnti-Caspase-10/CASP-10 antibody [EPR10890]
See all Caspase-10/CASP-10 primary antibodies
DescriptionRabbit monoclonal [EPR10890] to Caspase-10/CASP-10
Tested applicationsSuitable for: WB, IHC-P, IP, Flow Cytmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Recombinant fragment within Human Caspase-10/CASP-10. The exact sequence is proprietary.
Database link: Q92851
- K562 and Jurkat whole cell lysate (ab7899); Human hepatocellular carcinoma tissue; K562 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Caspase-10.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 58 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
-AMC and Asp-Glu-Val-Asp-
Isoform C is proteolytically inactive.
Tissue specificityDetectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
Involvement in diseaseDefects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
modificationsCleavage by granzyme B and autocatalytic activity generate the two active subunits.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- ALPS2 antibody
- Apoptotic protease Mch-4 antibody
- CASP 10 antibody
All lanes : Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 58 kDa
Western blot analysis on immunoprecipitation pellet from (1) K562 cell lysate or (2) 1XPBS (negative control) using ab177475 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caspase-10/CASP-10 with ab177475 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow cytometric analysis of permeabilized K562 cells labeling Caspase 10/CASP-10 with ab177475 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
ab177475 has been referenced in 3 publications.
- Yu CC et al. YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo-/radio-resistance by suppressing caspase-mediated apoptosis. J Pathol N/A:N/A (2019). PubMed: 30945298
- Xiao F et al. Sex-dependent aortic valve pathology in patients with rheumatic heart disease. PLoS One 12:e0180230 (2017). IHC-P ; Human . PubMed: 28662157
- Guo W et al. Role of caspase-10 in the death of acute leukemia cells. Oncol Lett 12:1623-1629 (2016). WB ; Human . PubMed: 27446483