Product nameAnti-Caspase-11 antibody
See all Caspase-11 primary antibodies
DescriptionRabbit polyclonal to Caspase-11
Specificityab22684 recognises caspase-11 proenzyme (43 kD) and the large (23 kD) and small (10 kD) subunits of the mature enzyme. No crossreactivity with other caspases tested: mouse caspases-1 and 3, human caspases 1 to 10 and 14.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Does not react with: Human
Recombinant full length protein corresponding to Mouse Caspase-11.
Database link: P70343
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Our Abpromise guarantee covers the use of ab22684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/15000. Predicted molecular weight: 43, 23 , 10 kDa.|
FunctionInvolved in ER-stress induced apoptosis. Cleaves caspase-1 (By similarity). Involved in the activation cascade of caspases responsible for apoptosis execution. Promotes IL-1 beta processing by ICE, so may also have a role in inflammatory responses.
Tissue specificityMostly expressed in lung and spleen; weaker in heart and lung; little in liver, skeletal muscle, kidney and testis. Not found in the brain.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
modificationsThe two subunits are derived from the precursor sequence by a autocatalytic mechanism.
Cellular localizationEndoplasmic reticulum membrane. Mitochondrion.
- Information by UniProt
- CASP-11 antibody
- CASP-4 antibody
- CASP11 antibody
All lanes : Anti-Caspase-11 antibody (ab22684) at 1/10000 dilution
Lane 1 : Mouse liver whole cell lysate
Lane 2 : Mouse hepatocyte whole cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43, 23 , 10 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
This product has been referenced in:
- Xu J et al. Macrophage endocytosis of high-mobility group box 1 triggers pyroptosis. Cell Death Differ N/A:N/A (2014). Read more (PubMed: 24769733) »
- Kang MJ et al. IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation. J Immunol 178:1948-59 (2007). WB ; Mouse . Read more (PubMed: 17237446) »