Overview

  • Product name
    Anti-Caspase-11 antibody [EPR18628]
    See all Caspase-11 primary antibodies
  • Description
    Rabbit monoclonal [EPR18628] to Caspase-11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse Caspase-11 aa 50-300. The exact sequence is proprietary.
    Database link: P70343

  • Positive control
    • WB: Untreated and treated with Lipopolysaccharide 10µg/ml for 8 hours RAW 264.7 whole cell lysate; YAC-1 and bEnd.3 whole cell lysates. IP: RAW 264.7 treated with Lipopolysaccharide 10µg/ml for 8 hours whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR18628
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 43, 35, 30 kDa (predicted molecular weight: 43 kDa).
IP 1/50.

Target

  • Function
    Involved in ER-stress induced apoptosis. Cleaves caspase-1 (By similarity). Involved in the activation cascade of caspases responsible for apoptosis execution. Promotes IL-1 beta processing by ICE, so may also have a role in inflammatory responses.
  • Tissue specificity
    Mostly expressed in lung and spleen; weaker in heart and lung; little in liver, skeletal muscle, kidney and testis. Not found in the brain.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The two subunits are derived from the precursor sequence by a autocatalytic mechanism.
  • Cellular localization
    Endoplasmic reticulum membrane. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP-11 antibody
    • CASP-4 antibody
    • CASP11 antibody
    • CASP4 antibody
    • CASP4_MOUSE antibody
    • Caspase 11 antibody
    • Caspase 11 apoptosis related cysteine peptidase antibody
    • Caspase 11 apoptosis related cysteine protease antibody
    • Caspase 4 antibody
    • Caspase 4 apoptosis related cysteine peptidase antibody
    • Caspase-11 antibody
    • Caspase-4 subunit p20 antibody
    • CASPL antibody
    • Ich 3 antibody
    • Ich3 antibody
    • ICH3 protease antibody
    • Protease ICH-3 antibody
    see all

Images

  • All lanes : Anti-Caspase-11 antibody [EPR18628] (ab180673) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with Lipopolysaccharide 10µg/ml for 8 hours whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 25,35,43 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Caspase-11 antibody [EPR18628] (ab180673) at 1/1000 dilution

    Lane 1 : YAC-1 (Mouse lymphoma cell line) whole cell lysate
    Lane 2 : bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 25,35,43 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes. 

  • Caspase-11 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate with ab180673 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab180673 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate, 10µg (Input).

    Lane 2: ab180673 IP in RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate.

    Lane 3: Rabbit  IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab180673 in RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

This product has been referenced in:
  • de Carvalho RVH  et al. Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome. Cell Rep 26:429-437.e5 (2019). Read more (PubMed: 30625325) »
  • Wang J  et al. Caspase-11-dependent pyroptosis of lung epithelial cells protects from melioidosis while caspase-1 mediates macrophage pyroptosis and production of IL-18. PLoS Pathog 14:e1007105 (2018). Read more (PubMed: 29791511) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Treatment
100 ng/ml LPS
Specification
mouse hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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Verified customer

Submitted Dec 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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