Recombinant Anti-Caspase-11 antibody [EPR18628] (ab180673)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18628] to Caspase-11
- Suitable for: WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Caspase-11 antibody [EPR18628]
See all Caspase-11 primary antibodies -
Description
Rabbit monoclonal [EPR18628] to Caspase-11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Untreated and treated with Lipopolysaccharide 10µg/ml for 8 hours RAW 264.7 whole cell lysate; YAC-1 and bEnd.3 whole cell lysates. IP: RAW 264.7 treated with Lipopolysaccharide 10µg/ml for 8 hours whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18628 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Detects a band of approximately 43, 35, 30 kDa (predicted molecular weight: 43 kDa).
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IP |
1/50.
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Notes |
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WB
1/1000. Detects a band of approximately 43, 35, 30 kDa (predicted molecular weight: 43 kDa). |
IP
1/50. |
Target
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Function
Involved in ER-stress induced apoptosis. Cleaves caspase-1 (By similarity). Involved in the activation cascade of caspases responsible for apoptosis execution. Promotes IL-1 beta processing by ICE, so may also have a role in inflammatory responses. -
Tissue specificity
Mostly expressed in lung and spleen; weaker in heart and lung; little in liver, skeletal muscle, kidney and testis. Not found in the brain. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by a autocatalytic mechanism. -
Cellular localization
Endoplasmic reticulum membrane. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 12363 Mouse
- SwissProt: P70343 Mouse
- Unigene: 1569 Mouse
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Alternative names
- CASP-11 antibody
- CASP-4 antibody
- CASP11 antibody
see all
Images
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All lanes : Anti-Caspase-11 antibody [EPR18628] (ab180673) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with Lipopolysaccharide 10µg/ml for 8 hours whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 25,35,43 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Caspase-11 antibody [EPR18628] (ab180673) at 1/1000 dilution
Lane 1 : YAC-1 (Mouse lymphoma cell line) whole cell lysate
Lane 2 : bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 25,35,43 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes.
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Caspase-11 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate with ab180673 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab180673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate, 10µg (Input).
Lane 2: ab180673 IP in RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab180673 in RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (27)
ab180673 has been referenced in 27 publications.
- Fu H et al. Activating α7nAChR ameliorates abdominal aortic aneurysm through inhibiting pyroptosis mediated by NLRP3 inflammasome. Acta Pharmacol Sin 43:2585-2595 (2022). PubMed: 35217818
- Melhem H et al. Epithelial GPR35 protects from Citrobacter rodentium infection by preserving goblet cells and mucosal barrier integrity. Mucosal Immunol 15:443-458 (2022). PubMed: 35264769
- Wen H et al. Ruscogenins Improve CD-Like Enteritis by Inhibiting Apoptosis of Intestinal Epithelial Cells and Activating Nrf2/NQO1 Pathway. Oxid Med Cell Longev 2022:4877275 (2022). PubMed: 35308175
- Liang H et al. Pyolysin of Trueperella pyogenes Induces Pyroptosis and IL-1β Release in Murine Macrophages Through Potassium/NLRP3/Caspase-1/Gasdermin D Pathway. Front Immunol 13:832458 (2022). PubMed: 35371034
- Talà A et al. HrpA anchors meningococci to the dynein motor and affects the balance between apoptosis and pyroptosis. J Biomed Sci 29:45 (2022). PubMed: 35765029