Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18628] to Caspase-11 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse
Product nameAnti-Caspase-11 antibody [EPR18628] - BSA and Azide free
See all Caspase-11 primary antibodies
DescriptionRabbit monoclonal [EPR18628] to Caspase-11 - BSA and Azide free
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse Caspase-11 aa 50-300. The exact sequence is proprietary.
Database link: P70343
- IP: RAW 264.7 treated with Lipopolysaccharide 10µg/ml for 8 hours whole cell lysate.
Ab240991 is the carrier-free version of ab180673. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240991 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab240991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 43, 35, 30 kDa (predicted molecular weight: 43 kDa).|
FunctionInvolved in ER-stress induced apoptosis. Cleaves caspase-1 (By similarity). Involved in the activation cascade of caspases responsible for apoptosis execution. Promotes IL-1 beta processing by ICE, so may also have a role in inflammatory responses.
Tissue specificityMostly expressed in lung and spleen; weaker in heart and lung; little in liver, skeletal muscle, kidney and testis. Not found in the brain.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
modificationsThe two subunits are derived from the precursor sequence by a autocatalytic mechanism.
Cellular localizationEndoplasmic reticulum membrane. Mitochondrion.
- Information by UniProt
- CASP-11 antibody
- CASP-4 antibody
- CASP11 antibody
Caspase-11 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate with ab180673 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab180673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate, 10µg (Input).
Lane 2: ab180673 IP in RAW 264.7 treated with Lipopolysaccharide 10μg/ml for 8 hours whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180673).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240991 has not yet been referenced specifically in any publications.