Overview

  • Product name

    Caspase-12 (active) FITC Staining Kit
    See all Caspase-12 kits
  • Detection method

    Fluorescent
  • Assay type

    Enzyme activity
  • Product overview

    The Caspase-12 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase-12 in living cells. The assay utilizes the caspase-12 inhibitor, ATAD-FMK, conjugated to FITC (FITC-ATAD-FMK) as a marker. FITC-ATAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-12 in apoptotic cells. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

  • Notes

    Other caspase and apoptosis assays

    Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

Associated products

Protocols

References

This product has been referenced in:

  • Ryan SD  et al. Neuronal dystonin isoform 2 is a mediator of endoplasmic reticulum structure and function. Mol Biol Cell 23:553-66 (2012). Read more (PubMed: 22190742) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

The developer of the kit suggests trypsinizing the cells and then using the protocol for cells in suspension. If you do the assay this way, it would probably be best any apply the apoptosis-inducing treatment to the cells after trypsinization, to the cells in suspension. However, I have not seen a reference or data for this, so I do not know if the trypsinization will itself affect the caspase cascade.

If you go with what we discussed, treating and staining the cells while adhered, you should consider the possibility that some of the apoptotic cells may detach from the culture plate or flask as a consequence of the apoptosis-inducing treatment. (I am assuming you are comparing cells +/- treatment). If this is the case, treating and staining trypsinized cells might be a better approach, so that none of the cells are lost (that is, the detached, dying cells) during the assay.

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