Overview

  • Product name

  • Description

    Rabbit polyclonal to Caspase-12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • ICC/IF: NIH/3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab235180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 15 µg/ml.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 38 kDa.

Target

  • Function

    Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.
  • Tissue specificity

    Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis.
  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • CASP 12 antibody
    • CASP-12 antibody
    • Casp12 antibody
    • CASP12P1 antibody
    • caspase 12 (gene/pseudogene) antibody
    • caspase 12 pseudogene 1 antibody
    • CASPC_HUMAN antibody
    • Inactive caspase-12 antibody
    • OTTHUMP00000207032 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling Caspase-12 with ab235180 at 15 µg/ml , followed by Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml for 1 hour at RT (green).

    Expected localization: Endoplasmic reticulum. The nuclear counter stain is DAPI (blue).

    Panel A: DAPI.

    Panel B: ab235180 + secondary antibody.

    Panel C: Merge of panel A and B.

    Panel D: Secondary only.

     

  • Anti-Caspase-12 antibody (ab235180) at 0.5 µg/ml + NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 35 µg

    Secondary
    Goat anti-rabbit IgG [H&L] HRP at 1/40000 dilution

    Predicted band size: 38 kDa


    Exposure time: 30 seconds

References

ab235180 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235180.
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