Key features and details
- Rabbit polyclonal to Caspase-12
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Caspase-12 antibody
See all Caspase-12 primary antibodies
DescriptionRabbit polyclonal to Caspase-12
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide. This information is considered to be commercially sensitive.
- ICC/IF: NIH/3T3 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 15 µg/ml.|
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 38 kDa.|
FunctionHas no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.
Tissue specificityDetected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
- Information by UniProt
- CASP 12 antibody
- CASP-12 antibody
- Casp12 antibody
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling Caspase-12 with ab235180 at 15 µg/ml , followed by Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml for 1 hour at RT (green).
Expected localization: Endoplasmic reticulum. The nuclear counter stain is DAPI (blue).
Panel A: DAPI.
Panel B: ab235180 + secondary antibody.
Panel C: Merge of panel A and B.
Panel D: Secondary only.
Anti-Caspase-12 antibody (ab235180) at 0.5 µg/ml + NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 35 µg
Goat anti-rabbit IgG [H&L] HRP at 1/40000 dilution
Predicted band size: 38 kDa
Exposure time: 30 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235180 has not yet been referenced specifically in any publications.